Search Results
Overview
| Uniprot ID | Q5PPJ6 |
|---|---|
| Protein Name | Leucine--tRNA ligase, cytoplasmic |
| Gene Name | Lars1 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 923 | KNYMTPAKGKKTDKQ |
Function
Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)
Protein Sequence
10
MAERKGTAKV
20
DFLKKIEKEV
30
QQKWEAEKVF
40
EVSASSLEKQ
50
KQSSKGKYFV
60
TFPYPYMNGR
70
LHLGHTFSLS
80
KCEFAVGYQR
90
LKGKSCLFPF
100
GLHCTGMPIK
110
ACADKLKREI
120
ELYGCPPDFP
130
EEEEEEEESS
140
AKPGDIVMKD
150
KAKGKKSKAA
160
AKAGSSKYQW
170
NIMKSLGLSD
180
EDIAKFSEAE
190
HWLDYFPPLA
200
VQDLKKIGLK
210
VDWRRSFITT
220
DVNPYYDSFV
230
RWQFLTLRER
240
NKIKFGKRYT
250
IYSPKDGQPC
260
MDHDRQTGEG
270
VGPQEYTLVK
280
LKVLDPYPSK
290
LSGLKGKNIF
300
LVAATLRPET
310
MFGQTNCWVR
320
PDMKYIGFET
330
ANGDIFICTQ
340
RAARNMSYQG
350
FTKHNGVVPV
360
VKELMGEEIL
370
GASLSAPLTC
380
YKVIYVLPML
390
TIKEDKGTGV
400
VTSVPSDSPD
410
DFAALRDLKK
420
KQALRAKYAV
430
RDDMVLPFEP
440
VPVLEIPGIG
450
NLPAVTVCDE
460
LKIQSQNDRE
470
KLAEAKEKLY
480
LRGFYDGVML
490
VDGFKGQKIQ
500
HVKKTIQKNM
510
IDAGDALIYM
520
EPEKQVVSRS
530
ADECVVALCD
540
QWYLDYGDEN
550
WKKQTFQCLK
560
NMETFCEESR
570
KNFEASLDWL
580
QEHACSRTYG
590
LGTRLPWDEQ
600
WLIESLSDST
610
IYMAFYTVAH
620
LLQGGDLQGQ
630
AESPLGIRPQ
640
QMTRDVWDYV
650
FFKDAPFPKT
660
QIPKEKLDQL
670
KQEFEFWYPV
680
DLRASGKDLI
690
PNHLSYYIYN
700
HVAMWPEQSD
710
KWPVSVRANG
720
HLLLNSEKMS
730
KSTGNFLTLS
740
QAVDKFSADG
750
MRLALADAGD
760
TVEDANFVEA
770
MADAGILRLY
780
TWVEWVKEML
790
ANCSSLRSGP
800
ANSFNDRVFA
810
SEMNAGIIKT
820
DQNYEKMMFK
830
EALKTGFFEF
840
QAAKDKYREL
850
ATEGMHRELV
860
FRFIEVQTIL
870
LTPFCPHLCE
880
HIWTLLGKPD
890
SIMHASWPVA
900
GPVDESLIRS
910
SQYLMEVAHD
920
LRLRLKNYMT
930
PAKGKKTDKQ
940
PVQRPSHCTI
950
YVAKNYPVWQ
960
HITLTTLRSH
970
FEANNGKLPD
980
NKVIACELGS
990
LPELKKYMKK
1000
VMPFVAMIKE
1010
NMEKKGPRVL
1020
DLELEFDEQA
1030
VLMENIVYLT
1040
NSLELEHIDV
1050
KFASEAEDKV
1060
REECCPGKPL
1070
NVFRTEPGVP
1080
VSLVNPQPSS
1090
GHFSTKIDIR
1100
QGDSCESIIR
1110
RLTKMDRGIK
1120
DLSKVKLMRF
1130
DDPLLGPRRV
1140
PVLGREQNEK
1150
TLISENAIFH
1160
VDLVSKKVHL
1170
TENGLRADIG
DTIVYLVH
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0017101 | aminoacyl-tRNA synthetase multienzyme complex |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0012505 | endomembrane system |
| Cellular Component | GO:0005783 | endoplasmic reticulum |
| Cellular Component | GO:0005764 | lysosome |
| Molecular Function | GO:0002161 | aminoacyl-tRNA deacylase activity |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0004819 | glutamine-tRNA ligase activity |
| Molecular Function | GO:0005096 | GTPase activator activity |
| Molecular Function | GO:0004823 | leucine-tRNA ligase activity |
| Biological Process | GO:0034198 | cellular response to amino acid starvation |
| Biological Process | GO:0071230 | cellular response to amino acid stimulus |
| Biological Process | GO:0071233 | cellular response to L-leucine |
| Biological Process | GO:1990253 | cellular response to leucine starvation |
| Biological Process | GO:0006425 | glutaminyl-tRNA aminoacylation |
| Biological Process | GO:0006429 | leucyl-tRNA aminoacylation |
| Biological Process | GO:1904263 | positive regulation of TORC1 signaling |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.