Overview
| Uniprot ID | Q5RK09 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit G |
|---|
| Gene Name | Eif3g |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 153 |
MNKLKGQKIVSCRIC |
| 209 |
PVQAAQNKTGKYVPP |
| 212 |
AAQNKTGKYVPPSLR |
Function
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA
Protein Sequence
10
MPTGDFDSKP
20
SWADQVEEEG
30
EDDKCVTSEL
40
LKGIPLPTGD
50
TSPEPELLPG
60
DPLPPPKEVI
70
NGNIKTVTEY
80
KVEEDGKKFK
90
IVRTFRIETR
100
KASKAVARRK
110
NWKKFGNSEF
120
DPPGPNVATT
130
TVSDDVSMTF
140
ITSKEDLNCQ
150
EEEDPMNKLK
160
GQKIVSCRIC
170
KGDHWTTRCP
180
YKDTLGPMQK
190
ELAEQLGLST
200
GEKEKLPGEL
210
EPVQAAQNKT
220
GKYVPPSLRD
230
GASRRGESMQ
240
PNRRADDNAT
250
IRVTNLSEDT
260
RETDLQELFR
270
PFGSISRIYL
280
AKDKTTGQSK
290
GFAFISFHRR
300
EDAARAIAGV
310
SGFGYDHLIL
320
NVEWAKPSTN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
| Biological Process |
GO:0006413 |
translational initiation |
| Biological Process |
GO:0075525 |
viral translational termination-reinitiation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.