Search Results

Overview

Uniprot IDQ5T481
Protein NameRNA-binding protein 20
Gene NameRBM20
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
372 GGRLNNSKQGFIGAG

Function

RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed:22466703, PubMed:24960161, PubMed:26604136, PubMed:27496873, PubMed:27531932, PubMed:29895960, PubMed:30948719, PubMed:32840935, PubMed:34732726, PubMed:35427468). Acts as a repressor of mRNA splicing: specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed:24960161, PubMed:30948719, PubMed:34732726). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed:27531932, PubMed:30948719). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed:27531932, PubMed:34732726). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity)

Protein Sequence

10 MVLAAAMSQD 20 ADPSGPEQPD 30 RVACSVPGAR 40 ASPAPSGPRG 50 MQQPPPPPQP 60 PPPPQAGLPQ 70 IIQNAAKLLD 80 KNPFSVSNPN 90 PLLPSPASLQ 100 LAQLQAQLTL 110 HRLKLAQTAV 120 TNNTAAATVL 130 NQVLSKVAMS 140 QPLFNQLRHP 150 SVITGPHGHA 160 GVPQHAAAIP 170 STRFPSNAIA 180 FSPPSQTRGP 190 GPSMNLPNQP 200 PSAMVMHPFT 210 GVMPQTPGQP 220 AVILGIGKTG 230 PAPATAGFYE 240 YGKASSGQTY 250 GPETDGQPGF 260 LPSSASTSGS 270 VTYEGHYSHT 280 GQDGQAAFSK 290 DFYGPNSQGS 300 HVASGFPAEQ 310 AGGLKSEVGP 320 LLQGTNSQWE 330 SPHGFSGQSK 340 PDLTAGPMWP 350 PPHNQPYELY 360 DPEEPTSDRT 370 PPSFGGRLNN 380 SKQGFIGAGR 390 RAKEDQALLS 400 VRPLQAHELN 410 DFHGVAPLHL 420 PHICSICDKK 430 VFDLKDWELH 440 VKGKLHAQKC 450 LVFSENAGIR 460 CILGSAEGTL 470 CASPNSTAVY 480 NPAGNEDYAS 490 NLGTSYVPIP 500 ARSFTQSSPT 510 FPLASVGTTF 520 AQRKGAGRVV 530 HICNLPEGSC 540 TENDVINLGL 550 PFGKVTNYIL 560 MKSTNQAFLE 570 MAYTEAAQAM 580 VQYYQEKSAV 590 INGEKLLIRM 600 SKRYKELQLK 610 KPGKAVAAII 620 QDIHSQRERD 630 MFREADRYGP 640 ERPRSRSPVS 650 RSLSPRSHTP 660 SFTSCSSSHS 670 PPGPSRADWG 680 NGRDSWEHSP 690 YARREEERDP 700 APWRDNGDDK 710 RDRMDPWAHD 720 RKHHPRQLDK 730 AELDERPEGG 740 RPHREKYPRS 750 GSPNLPHSVS 760 SYKSREDGYY 770 RKEPKAKSDK 780 YLKQQQDAPG 790 RSRRKDEARL 800 RESRHPHPDD 810 SGKEDGLGPK 820 VTRAPEGAKA 830 KQNEKNKTKR 840 TDRDQEGADD 850 RKENTMAENE 860 AGKEEQEGME 870 ESPQSVGRQE 880 KEAEFSDPEN 890 TRTKKEQDWE 900 SESEAEGESW 910 YPTNMEELVT 920 VDEVGEEEDF 930 IVEPDIPELE 940 EIVPIDQKDK 950 ICPETCLCVT 960 TTLDLDLAQD 970 FPKEGVKAVG 980 NGAAEISLKS 990 PRELPSASTS 1000 CPSDMDVEMP 1010 GLNLDAERKP 1020 AESETGLSLE 1030 DSDCYEKEAK 1040 GVESSDVHPA 1050 PTVQQMSSPK 1060 PAEERARQPS 1070 PFVDDCKTRG 1080 TPEDGACEGS 1090 PLEEKASPPI 1100 ETDLQNQACQ 1110 EVLTPENSRY 1120 VEMKSLEVRS 1130 PEYTEVELKQ 1140 PLSLPSWEPE 1150 DVFSELSIPL 1160 GVEFVVPRTG 1170 FYCKLCGLFY 1180 TSEETAKMSH 1190 CRSAVHYRNL 1200 QKYLSQLAEE 1210 GLKETEGADS 1220 PRPEDSGIVP RFERKKL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0036464 cytoplasmic ribonucleoprotein granule
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0097157 pre-mRNA intronic binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:1990935 splicing factor binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0060914 heart formation
Biological Process GO:0048025 negative regulation of mRNA splicing, via spliceosome
Biological Process GO:0033120 positive regulation of RNA splicing
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:0048024 regulation of mRNA splicing, via spliceosome
Biological Process GO:0043484 regulation of RNA splicing
Biological Process GO:0160091 spliceosome-depend formation of circular RNA

Reference

PMID: N/A