Search Results

Overview

Uniprot ID	Q5T5Y3
Protein Name	Calmodulin-regulated spectrin-associated protein 1
Gene Name	CAMSAP1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1315	LEAEVELKRDEARRK
222	LESPAHQKVRYRREH
49	NLQWICAKAYGRDNI

Function

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19508979, PubMed:21834987, PubMed:24117850, PubMed:24486153, PubMed:24706919). Specifically recognizes growing microtubule minus-ends and stabilizes microtubules (PubMed:24486153, PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919). In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation (PubMed:24486153, PubMed:24706919). Through interaction with spectrin may regulate neurite outgrowth (PubMed:24117850)

Protein Sequence

10 MVDASGRAAA 20 EGWRKMEAPP 30 DGAADLVPLD 40 RYDAARAKIA 50 ANLQWICAKA 60 YGRDNIPEDL 70 RDPFYVDQYE 80 QEHIKPPVIK 90 LLLSSELYCR 100 VCSLILKGDQ 110 VAALQGHQSV 120 IQALSRKGIY 130 VMESDDTPVT 140 ESDLSRAPIK 150 MSAHMAMVDA 160 LMMAYTVEMI 170 SIEKVVASVK 180 RFSTFSASKE 190 LPYDLEDAMV 200 FWINKVNLKM 210 REITEKEVKL 220 KQQLLESPAH 230 QKVRYRREHL 240 SARQSPYFPL 250 LEDLMRDGSD 260 GAALLAVIHY 270 YCPEQMKLDD 280 ICLKEVTSMA 290 DSLYNIRLLR 300 EFSNEYLNKC 310 FYLTLEDMLY 320 APLVLKPNVM 330 VFIAELFWWF 340 ENVKPDFVQP 350 RDVQELKDAK 360 TVLHQKSSRP 370 PVPISNATKR 380 SFLGSPAAGT 390 LAELQPPVQL 400 PAEGCHRHYL 410 HPEEPEYLGK 420 GTAAFSPSHP 430 LLPLRQKQQK 440 SIQGEDIPDQ 450 RHRSNSLTRV 460 DGQPRGAAIA 470 WPEKKTRPAS 480 QPTPFALHHA 490 ASCEVDPSSG 500 DSISLARSIS 510 KDSLASNIVN 520 LTPQNQPHPT 530 ATKSHGKSLL 540 SNVSIEDEEE 550 ELVAIVRADV 560 VPQQADPEFP 570 RASPRALGLT 580 ANARSPQGQL 590 DTSESKPDSF 600 FLEPLMPAVL 610 KPAKEKQVIT 620 KEDERGEGRP 630 RSIVSRRPSE 640 GPQPLVRRKM 650 TGSRDLNRTF 660 TPIPCSEFPM 670 GIDPTETGPL 680 SVETAGEVCG 690 GPLALGGFDP 700 FPQGPSTDGF 710 FLHVGRADED 720 TEGRLYVSCS 730 KSPNSHDSEP 740 WTLLRQDSDS 750 DVVDIEEAEH 760 DFMGEAHPVV 770 FSRYIGEEES 780 AKLQEDMKVK 790 EHEDKDDASG 800 RSSPCLSTAS 810 QMSSVSMASG 820 SVKMTSFAER 830 KLQRLNSCET 840 KSSTSSSQKT 850 TPDASESCPA 860 PLTTWRQKRE 870 QSPSQHGKDP 880 ASLLASELVQ 890 LHMQLEEKRR 900 AIEAQKKKME 910 ALSARQRLKL 920 GKAAFLHVVK 930 KGKAEAAPPL 940 RPEHFAKEYS 950 QHNGEDCGDA 960 VSKTEDFLVK 970 EEQREELLHE 980 PQDVDKESLA 990 FAQQHKAKDP 1000 VALHELERNK 1010 VISAALLEDT 1020 VGEVVDVNEC 1030 DLSIEKLNET 1040 ISTLQQAILK 1050 ISQQQEQLLM 1060 KSPTVPVPGS 1070 KNNSQDHKVK 1080 APVHFVEPLS 1090 PTGVAGHRKA 1100 PRLGQGRNSR 1110 SGRPAELKVP 1120 KDRPQGSSRS 1130 KTPTPSVETL 1140 PHLRPFPASS 1150 HPRTPTDPGL 1160 DSALEPSGDP 1170 HGKCLFDSYR 1180 LHDESNQRTL 1190 TLSSSKDANI 1200 LSEQMSLKEV 1210 LDASVKEVGS 1220 SSSDVSGKES 1230 VPVEEPLRSR 1240 ASLIEVDLSD 1250 LKAPDEDGEL 1260 VSLDGSADLV 1270 SEGDQKPGVG 1280 FFFKDEQKAE 1290 DELAKKRAAF 1300 LLKQQRKAEE 1310 ARVRKQQLEA 1320 EVELKRDEAR 1330 RKAEEDRVRK 1340 EEEKARRELI 1350 KQEYLRRKQQ 1360 QILEEQGLGK 1370 PKSKPKKPRP 1380 KSVHREESCS 1390 DSGTKCSSTP 1400 DNLSRTQSGS 1410 SLSLASAATT 1420 EPESVHSGGT 1430 PSQRVESMEA 1440 LPILSRNPSR 1450 STDRDWETAS 1460 AASSLASVAE 1470 YTGPKLFKEP 1480 SSKSNKPIIH 1490 NAISHCCLAG 1500 KVNEPHKNSI 1510 LEELEKCDAN 1520 HYIILFRDAG 1530 CQFRALYCYY 1540 PDTEEIYKLT 1550 GTGPKNITKK 1560 MIDKLYKYSS 1570 DRKQFNLIPA 1580 KTMSVSVDAL 1590 TIHNHLWQPK 1600 RPAVPKKAQT RK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005874	microtubule
Cellular Component	GO:0072686	mitotic spindle
Molecular Function	GO:0005516	calmodulin binding
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0051011	microtubule minus-end binding
Molecular Function	GO:0030507	spectrin binding
Biological Process	GO:0000226	microtubule cytoskeleton organization
Biological Process	GO:0031175	neuron projection development
Biological Process	GO:0031113	regulation of microtubule polymerization

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.