Overview
| Uniprot ID | Q5TC82 |
|---|
| Protein Name | Roquin-1 |
|---|
| Gene Name | RC3H1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 239 |
PRFPQASKTSIGHVV |
Function
Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF, TNFRSF4 and in many more mRNAs (PubMed:25026078, PubMed:31636267). Cleaves translationally inactive mRNAs harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-independent manner (By similarity). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity (By similarity). In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression (By similarity). Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation (By similarity). Recognizes and binds mRNAs containing a hexaloop stem-loop motif, called alternative decay element (ADE) (By similarity). Together with ZC3H12A, destabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR (By similarity). Able to interact with double-stranded RNA (dsRNA) (PubMed:25026078, PubMed:25504471). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression (PubMed:25697406, PubMed:31636267). Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2A, UBE2B, UBE2D2, UBE2F, UBE2G1, UBE2G2 and UBE2L3 and produces polyubiquitin chains (PubMed:26489670). Shows the strongest activity when paired with UBE2N:UBE2V1 or UBE2N:UBE2V2 E2 complexes and generate both short and long polyubiquitin chains (PubMed:26489670)
Protein Sequence
10
MPVQAPQWTD
20
FLSCPICTQT
30
FDETIRKPIS
40
LGCGHTVCKM
50
CLNKLHRKAC
60
PFDQTTINTD
70
IELLPVNSAL
80
LQLVGAQVPE
90
QQPITLCSGV
100
EDTKHYEEAK
110
KCVEELALYL
120
KPLSSARGVG
130
LNSTTQSVLS
140
RPMQRKLVTL
150
VHCQLVEEEG
160
RIRAMRAARS
170
LGERTVTELI
180
LQHQNPQQLS
190
SNLWAAVRAR
200
GCQFLGPAMQ
210
EEALKLVLLA
220
LEDGSALSRK
230
VLVLFVVQRL
240
EPRFPQASKT
250
SIGHVVQLLY
260
RASCFKVTKR
270
DEDSSLMQLK
280
EEFRTYEALR
290
REHDSQIVQI
300
AMEAGLRIAP
310
DQWSSLLYGD
320
QSHKSHMQSI
330
IDKLQTPASF
340
AQSVQELTIA
350
LQRTGDPANL
360
NRLRPHLELL
370
ANIDPSPDAP
380
PPTWEQLENG
390
LVAVRTVVHG
400
LVDYIQNHSK
410
KGADQQQPPQ
420
HSKYKTYMCR
430
DMKQRGGCPR
440
GASCTFAHSQ
450
EELEKFRKMN
460
KRLVPRRPLS
470
ASLGQLNEVG
480
LPSAAILPDE
490
GAVDLPSRKP
500
PALPNGIVST
510
GNTVTQLIPR
520
GTDPSYDSSL
530
KPGKIDHLSS
540
SAPGSPPDLL
550
ESVPKSISAL
560
PVNPHSIPPR
570
GPADLPPMPV
580
TKPLQMVPRG
590
SQLYPAQQTD
600
VYYQDPRGAA
610
PPFEPAPYQQ
620
GMYYTPPPQC
630
VSRFVRPPPS
640
APEPAPPYLD
650
HYPPYLQERV
660
VNSQYGTQPQ
670
QYPPIYPSHY
680
DGRRVYPAPS
690
YTREEIFRES
700
PIPIEIPPAA
710
VPSYVPESRE
720
RYQQIESYYP
730
VAPHPTQIRP
740
SYLREPPYSR
750
LPPPPQPHPS
760
LDELHRRRKE
770
IMAQLEERKV
780
ISPPPFAPSP
790
TLPPTFHPEE
800
FLDEDLKVAG
810
KYKGNDYSQY
820
SPWSCDTIGS
830
YIGTKDAKPK
840
DVVAAGSVEM
850
MNVESKGMRD
860
QRLDLQRRAA
870
ETSDDDLIPF
880
GDRPTVSRFG
890
AISRTSKTIY
900
QGAGPMQAMA
910
PQGAPTKSIN
920
ISDYSPYGTH
930
GGWGASPYSP
940
HQNIPSQGHF
950
SERERISMSE
960
VASHGKPLPS
970
AEREQLRLEL
980
QQLNHQISQQ
990
TQLRGLEAVS
1000
NRLVLQREAN
1010
TLAGQSQPPP
1020
PPPPKWPGMI
1030
SSEQLSLELH
1040
QVEREIGKRT
1050
RELSMENQCS
1060
LDMKSKLNTS
1070
KQAENGQPEP
1080
QNKVPAEDLT
1090
LTFSDVPNGS
1100
ALTQENISLL
1110
SNKTSSLNLS
1120
EDPEGGGDNN
1130
DSQRSGVTPS
SAP
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0045623 |
negative regulation of T-helper cell differentiation |
| Cellular Component |
GO:0010494 |
cytoplasmic stress granule |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0000932 |
P-body |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:1905762 |
CCR4-NOT complex binding |
| Molecular Function |
GO:0003725 |
double-stranded RNA binding |
| Molecular Function |
GO:0035198 |
miRNA binding |
| Molecular Function |
GO:0003730 |
mRNA 3'-UTR binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0035613 |
RNA stem-loop binding |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0004842 |
ubiquitin-protein transferase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0061158 |
3'-UTR-mediated mRNA destabilization |
| Biological Process |
GO:0071347 |
cellular response to interleukin-1 |
| Biological Process |
GO:0046007 |
negative regulation of activated T cell proliferation |
| Biological Process |
GO:0030889 |
negative regulation of B cell proliferation |
| Biological Process |
GO:0002635 |
negative regulation of germinal center formation |
| Biological Process |
GO:2000320 |
negative regulation of T-helper 17 cell differentiation |
| Biological Process |
GO:0000956 |
nuclear-transcribed mRNA catabolic process |
| Biological Process |
GO:0000288 |
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay |
| Biological Process |
GO:0000184 |
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay |
| Biological Process |
GO:0033962 |
P-body assembly |
| Biological Process |
GO:0010608 |
post-transcriptional regulation of gene expression |
| Biological Process |
GO:0000209 |
protein polyubiquitination |
| Biological Process |
GO:0002634 |
regulation of germinal center formation |
| Biological Process |
GO:2000628 |
regulation of miRNA metabolic process |
| Biological Process |
GO:0043488 |
regulation of mRNA stability |
| Biological Process |
GO:1900151 |
regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay |
| Biological Process |
GO:0050856 |
regulation of T cell receptor signaling pathway |
| Biological Process |
GO:0050852 |
T cell receptor signaling pathway |
| Biological Process |
GO:0006511 |
ubiquitin-dependent protein catabolic process |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.