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Overview

Uniprot IDQ5U300
Protein NameUbiquitin-like modifier-activating enzyme 1
Gene NameUba1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
185 FCHSRGIKLVVADTR
296 GGIVSQVKVPKKISF
528 PWDVTKLKSDTAAAA

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites

Protein Sequence

10 MSSSPLSKKR 20 RVSGPDPKPG 30 SNCSSAQSVL 40 SEVSSVPTNG 50 MAKNGSEADI 60 DESLYSRQLY 70 VLGHEAMKML 80 QTSSVLVSGL 90 RGLGVEIAKN 100 IILGGVKAVT 110 LHDQGTTQWA 120 DLSSQFYLRE 130 EDIGKNRAEV 140 SQPRLAELNS 150 YVPVTAYTGP 160 LVEDFLSGFQ 170 VVVLTNSPLE 180 EQLRVGEFCH 190 SRGIKLVVAD 200 TRGLFGQLFC 210 DFGEEMVLTD 220 SNGEQPLSAM 230 VSMVTKDNPG 240 VVTCLDEARH 250 GFETGDFVSF 260 SEVQGMVQLN 270 GCQPIEIKVL 280 GPYTFSICDT 290 SNFSDYIRGG 300 IVSQVKVPKK 310 ISFKSLPASL 320 AEPDFVMTDF 330 AKYSRPAQLH 340 IGFQALHQFC 350 AQHNRPPRPR 360 NEEDATELVT 370 LAQAVNARSP 380 PAVQQDNVDE 390 DLIRKLAYVA 400 AGDLAPINAF 410 IGGLAAQEVM 420 KACSGKFMPI 430 MQWLYFDALE 440 CLPEDKEALT 450 EDKCLPRQNR 460 YDGQVAVFGS 470 DLQEKLGKQK 480 YFLVGAGAIG 490 CELLKNFAMI 500 GLGCGEGGEV 510 VVTDMDTIEK 520 SNLNRQFLFR 530 PWDVTKLKSD 540 TAAAAVRQMN 550 PYIQVTSHQN 560 RVGPDTERIY 570 DDDFFQNLDG 580 VANALDNVDA 590 RMYMDRRCVY 600 YRKPLLESGT 610 LGTKGNVQVV 620 IPFLTESYSS 630 SQDPPEKSIP 640 ICTLKNFPNA 650 IEHTLQWARD 660 EFEGLFKQPA 670 ENVNQYLTDS 680 KFVERTLRLA 690 GTQPLEVLEA 700 VQRSLVLQRP 710 QTWGDCVTWA 720 CHHWHTQYCN 730 NIRQLLHNFP 740 PDQLTSSGAP 750 FWSGPKRCPH 760 PLTFDVNNTL 770 HLDYVMAAAN 780 LFAQTYGLTG 790 SQDRAAVASL 800 LQSVQVPEFT 810 PKSGVKIHVS 820 DQELQSANAS 830 VDDSRLEELK 840 ATLPSPDKLP 850 GFKMYPIDFE 860 KDDDSNFHMD 870 FIVAASNLRA 880 ENYDISPADR 890 HKSKLIAGKI 900 IPAIATTTAA 910 VVGLVCLELY 920 KVVQGHQQLD 930 SYKNGFLNLA 940 LPFFGFSEPL 950 AAPRHQYYNQ 960 EWTLWDRFEV 970 QGLQPNGEEM 980 TLKQFLDYFK 990 TEHKLEITML 1000 SQGVSMLYSF 1010 FMPAAKLKER 1020 LDQPMTEIVS 1030 RVSKRKLGRH 1040 VRALVLELCC 1050 NDESGEDVEV PYVRYTIR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030057 desmosome
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0000792 heterochromatin
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0030867 rough endoplasmic reticulum membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004839 ubiquitin activating enzyme activity
Biological Process GO:0006974 DNA damage response
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.