Overview
| Uniprot ID | Q5U367 |
|---|
| Protein Name | Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 |
|---|
| Gene Name | Plod3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 218 |
LKLSLDHKSRIFQNL |
Function
Multifunctional enzyme that catalyzes a series of post-translational modifications on Lys residues in procollagen. Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (By similarity). Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (By similarity). Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the ADIPOQ collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. Essential for normal biosynthesis and secretion of type IV collagens. Essential for normal formation of basement membranes (By similarity)
Protein Sequence
10
MAASVPEPRL
20
LLLLLLLLPP
30
LPPVTSASDR
40
PRGANPVNPD
50
KLLVITVATA
60
ETEGYRRFLQ
70
SAEFFNYTVR
80
TLGLGQEWRG
90
GDVARTVGGG
100
QKVRWLKKEM
110
EKYASQEDMI
120
IMFVDSYDVI
130
LASSPTELLK
140
KFVQSGSHLL
150
FSAESFCWPD
160
WGLAEQYPEV
170
GVGKRFLNSG
180
GFIGFAPTIH
190
RIVRQWKYKD
200
DDDDQLFYTQ
210
LYLDPGLREK
220
LKLSLDHKSR
230
IFQNLNGALD
240
EVVLKFDQNR
250
VRIRNVAYDT
260
LPVVVHGNGP
270
TKLQLNYLGN
280
YVPNGWTPQG
290
GCGFCNLNRR
300
TLPGGQPPPR
310
VLLAVFVEQP
320
TPFLPRFLQR
330
LLLLDYPPDR
340
ISLFLHNNEV
350
YHEPHIADAW
360
PQLQDHFSAV
370
KLVGPEEALS
380
SGEARDMAMD
390
SCRQNPECEF
400
YFSLDADAVL
410
TNPETLRILI
420
EQNRKVIAPM
430
LSRHGKLWSN
440
FWGALSPDEY
450
YARSEDYVEL
460
VQRKRVGLWN
470
VPYISQAYVI
480
RGETLRTELP
490
EKEVFSSSDT
500
DPDMAFCRSV
510
RDKGIFLHLS
520
NQHEFGRLLS
530
TSHYDTDHLH
540
PDLWQIFDNP
550
VDWREQYIHE
560
NYSRALDGEG
570
LVEQPCPDVY
580
WFPLLTEQMC
590
DELVEEMEHY
600
GQWSGGRHED
610
SRLAGGYENV
620
PTVDIHMKQV
630
GYEDQWLQLL
640
RTYVGPMTEH
650
LFPGYHTKTR
660
AVMNFVVRYR
670
PDEQPSLRPH
680
HDSSTFTLNV
690
ALNHKGVDYE
700
GGGCRFLRYD
710
CRVSSPRKGW
720
ALLHPGRLTH
730
YHEGLPTTRG
740
TRYIMVSFVD
P
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Biological Process |
GO:0060425 |
lung morphogenesis |
| Biological Process |
GO:0021915 |
neural tube development |
| Biological Process |
GO:0017185 |
peptidyl-lysine hydroxylation |
| Biological Process |
GO:0180062 |
protein O-linked glycosylation via galactose |
| Biological Process |
GO:0042311 |
vasodilation |
| Cellular Component |
GO:0005788 |
endoplasmic reticulum lumen |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0031012 |
extracellular matrix |
| Cellular Component |
GO:0005615 |
extracellular space |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0005791 |
rough endoplasmic reticulum |
| Cellular Component |
GO:0005802 |
trans-Golgi network |
| Molecular Function |
GO:0005506 |
iron ion binding |
| Molecular Function |
GO:0031418 |
L-ascorbic acid binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0050211 |
procollagen galactosyltransferase activity |
| Molecular Function |
GO:0033823 |
procollagen glucosyltransferase activity |
| Molecular Function |
GO:0008475 |
procollagen-lysine 5-dioxygenase activity |
| Molecular Function |
GO:0036094 |
small molecule binding |
| Biological Process |
GO:0070831 |
basement membrane assembly |
| Biological Process |
GO:0032870 |
cellular response to hormone stimulus |
| Biological Process |
GO:0032964 |
collagen biosynthetic process |
| Biological Process |
GO:0030199 |
collagen fibril organization |
| Biological Process |
GO:0032963 |
collagen metabolic process |
| Biological Process |
GO:0001886 |
endothelial cell morphogenesis |
| Biological Process |
GO:0048730 |
epidermis morphogenesis |
| Biological Process |
GO:0046947 |
hydroxylysine biosynthetic process |
| Biological Process |
GO:0001701 |
in utero embryonic development |
| Biological Process |
GO:0008104 |
intracellular protein localization |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.