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Overview

Uniprot IDQ5VWG9
Protein NameTranscription initiation factor TFIID subunit 3
Gene NameTAF3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
771 RVGAGQDKIVISKVV
776 QDKIVISKVVPAPEA

Function

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473). TAF3 forms the TFIID-A module together with TAF5 and TBP (PubMed:33795473). Required in complex with TBPL2 for the differentiation of myoblasts into myocytes (PubMed:11438666). The TAF3-TBPL2 complex replaces TFIID at specific promoters at an early stage in the differentiation process (PubMed:11438666)

Protein Sequence

10 MCESYSRSLL 20 RVSVAQICQA 30 LGWDSVQLSA 40 CHLLTDVLQR 50 YLQQLGRGCH 60 RYSELYGRTD 70 PILDDVGEAF 80 QLMGVSLHEL 90 EDYIHNIEPV 100 TFPHQIPSFP 110 VSKNNVLQFP 120 QPGSKDAEER 130 KEYIPDYLPP 140 IVSSQEEEEE 150 EQVPTDGGTS 160 AEAMQVPLEE 170 DDELEEEEII 180 NDENFLGKRP 190 LDSPEAEELP 200 AMKRPRLLST 210 KGDTLDVVLL 220 EAREPLSSIN 230 TQKIPPMLSP 240 VHVQDSTDLA 250 PPSPEPPMLA 260 PVAKSQMPTA 270 KPLETKSFTP 280 KTKTKTSSPG 290 QKTKSPKTAQ 300 SPAMVGSPIR 310 SPKTVSKEKK 320 SPGRSKSPKS 330 PKSPKVTTHI 340 PQTPVRPETP 350 NRTPSATLSE 360 KISKETIQVK 370 QIQTPPDAGK 380 LNSENQPKKA 390 VVADKTIEAS 400 IDAVIARACA 410 EREPDPFEFS 420 SGSESEGDIF 430 TSPKRISGPE 440 CTTPKASTSA 450 NNFTKSGSTP 460 LPLSGGTSSS 470 DNSWTMDASI 480 DEVVRKAKLG 490 TPSNMPPNFP 500 YISSPSVSPP 510 TPEPLHKVYE 520 EKTKLPSSVE 530 VKKKLKKELK 540 TKMKKKEKQR 550 DREREKDKNK 560 DKSKEKDKVK 570 EKEKDKETGR 580 ETKYPWKEFL 590 KEEEADPYKF 600 KIKEFEDVDP 610 KVKLKDGLVR 620 KEKEKHKDKK 630 KDREKGKKDK 640 DKREKEKVKD 650 KGREDKMKAP 660 APPLVLPPKE 670 LALPLFSPAT 680 ASRVPAMLPS 690 LLPVLPEKLF 700 EEKEKVKEKE 710 KKKDKKEKKK 720 KKEKEKEKKE 730 KEREKEKRER 740 EKREKEKEKH 750 KHEKIKVEPV 760 ALAPSPVIPR 770 LTLRVGAGQD 780 KIVISKVVPA 790 PEAKPAPSQN 800 RPKTPPPAPA 810 PAPGPMLVSP 820 APVPLPLLAQ 830 AAAGPALLPS 840 PGPAASGASA 850 KAPVRSVVTE 860 TVSTYVIRDE 870 WGNQIWICPG 880 CNKPDDGSPM 890 IGCDDCDDWY 900 HWPCVGIMTA 910 PPEEMQWFCP 920 KCANKKKDKK HKKRKHRAH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0031965 nuclear membrane
Molecular Function GO:0140416 transcription regulator inhibitor activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0051457 maintenance of protein location in nucleus
Biological Process GO:0042789 mRNA transcription by RNA polymerase II
Biological Process GO:1901797 negative regulation of signal transduction by p53 class mediator
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0060261 positive regulation of transcription initiation by RNA polymerase II
Biological Process GO:0051123 RNA polymerase II preinitiation complex assembly
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005669 transcription factor TFIID complex
Molecular Function GO:0140002 histone H3K4me3 reader activity
Molecular Function GO:0002039 p53 binding
Molecular Function GO:0046982 protein heterodimerization activity
Molecular Function GO:0016251 RNA polymerase II general transcription initiation factor activity

Reference

[1] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.