Search Results

Overview

Uniprot ID	Q5VYS8
Protein Name	Terminal uridylyltransferase 7
Gene Name	TUT7
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
130	PVINRQRKDSFQENE

Function

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:18172165, PubMed:19703396, PubMed:22898984, PubMed:25480299, PubMed:25979828, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351)

Protein Sequence

10 MGDTAKPYFV 20 KRTKDRGTMD 30 DDDFRRGHPQ 40 QDYLIIDDHA 50 KGHGSKMEKG 60 LQKKKITPGN 70 YGNTPRKGPC 80 AVSSNPYAFK 90 NPIYSQPAWM 100 NDSHKDQSKR 110 WLSDEHTGNS 120 DNWREFKPGP 130 RIPVINRQRK 140 DSFQENEDGY 150 RWQDTRGCRT 160 VRRLFHKDLT 170 SLETTSEMEA 180 GSPENKKQRS 190 RPRKPRKTRN 200 EENEQDGDLE 210 GPVIDESVLS 220 TKELLGLQQA 230 EERLKRDCID 240 RLKRRPRNYP 250 TAKYTCRLCD 260 VLIESIAFAH 270 KHIKEKRHKK 280 NIKEKQEEEL 290 LTTLPPPTPS 300 QINAVGIAID 310 KVVQEFGLHN 320 ENLEQRLEIK 330 RIMENVFQHK 340 LPDCSLRLYG 350 SSCSRLGFKN 360 SDVNIDIQFP 370 AIMSQPDVLL 380 LVQECLKNSD 390 SFIDVDADFH 400 ARVPVVVCRE 410 KQSGLLCKVS 420 AGNENACLTT 430 KHLTALGKLE 440 PKLVPLVIAF 450 RYWAKLCSID 460 RPEEGGLPPY 470 VFALMAIFFL 480 QQRKEPLLPV 490 YLGSWIEGFS 500 LSKLGNFNLQ 510 DIEKDVVIWE 520 HTDSAAGDTG 530 ITKEEAPRET 540 PIKRGQVSLI 550 LDVKHQPSVP 560 VGQLWVELLR 570 FYALEFNLAD 580 LVISIRVKEL 590 VSRELKDWPK 600 KRIAIEDPYS 610 VKRNVARTLN 620 SQPVFEYILH 630 CLRTTYKYFA 640 LPHKITKSSL 650 LKPLNAITCI 660 SEHSKEVINH 670 HPDVQTKDDK 680 LKNSVLAQGP 690 GATSSAANTC 700 KVQPLTLKET 710 AESFGSPPKE 720 EMGNEHISVH 730 PENSDCIQAD 740 VNSDDYKGDK 750 VYHPETGRKN 760 EKEKVGRKGK 770 HLLTVDQKRG 780 EHVVCGSTRN 790 NESESTLDLE 800 GFQNPTAKEC 810 EGLATLDNKA 820 DLDGESTEGT 830 EELEDSLNHF 840 THSVQGQTSE 850 MIPSDEEEED 860 DEEEEEEEEP 870 RLTINQREDE 880 DGMANEDELD 890 NTYTGSGDED 900 ALSEEDDELG 910 EAAKYEDVKE 920 CGKHVERALL 930 VELNKISLKE 940 ENVCEEKNSP 950 VDQSDFFYEF 960 SKLIFTKGKS 970 PTVVCSLCKR 980 EGHLKKDCPE 990 DFKRIQLEPL 1000 PPLTPKFLNI 1010 LDQVCIQCYK 1020 DFSPTIIEDQ 1030 AREHIRQNLE 1040 SFIRQDFPGT 1050 KLSLFGSSKN 1060 GFGFKQSDLD 1070 VCMTINGLET 1080 AEGLDCVRTI 1090 EELARVLRKH 1100 SGLRNILPIT 1110 TAKVPIVKFF 1120 HLRSGLEVDI 1130 SLYNTLALHN 1140 TRLLSAYSAI 1150 DPRVKYLCYT 1160 MKVFTKMCDI 1170 GDASRGSLSS 1180 YAYTLMVLYF 1190 LQQRNPPVIP 1200 VLQEIYKGEK 1210 KPEIFVDGWN 1220 IYFFDQIDEL 1230 PTYWSECGKN 1240 TESVGQLWLG 1250 LLRFYTEEFD 1260 FKEHVISIRR 1270 KSLLTTFKKQ 1280 WTSKYIVIED 1290 PFDLNHNLGA 1300 GLSRKMTNFI 1310 MKAFINGRRV 1320 FGIPVKGFPK 1330 DYPSKMEYFF 1340 DPDVLTEGEL 1350 APNDRCCRIC 1360 GKIGHFMKDC 1370 PMRRKVRRRR 1380 DQEDALNQRY 1390 PENKEKRSKE 1400 DKEIHNKYTE 1410 REVSTKEDKP 1420 IQCTPQKAKP 1430 MRAAADLGRE 1440 KILRPPVEKW 1450 KRQDDKDLRE 1460 KRCFICGREG 1470 HIKKECPQFK 1480 GSSGSLSSKY 1490 MTQGKASAKR TQQES

Gene Ontology

Classification	GO ID	Description
Biological Process	GO:0031123	RNA 3'-end processing
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0035198	miRNA binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0050265	RNA uridylyltransferase activity
Molecular Function	GO:0070569	uridylyltransferase activity
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0010586	miRNA metabolic process
Biological Process	GO:0001556	oocyte maturation
Biological Process	GO:1990074	polyuridylation-dependent mRNA catabolic process
Biological Process	GO:0031054	pre-miRNA processing
Biological Process	GO:0141008	transposable element silencing by mRNA destabilization

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.