Overview
| Uniprot ID | Q5W9D5 |
|---|
| Protein Name | KH domain-containing RNA-binding protein QKI |
|---|
| Gene Name | QKI |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 111 |
GPRGLTAKQLEAETG |
Function
RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, mRNA stability and/or translation. Involved in various cellular processes, such as mRNA storage into stress granules, apoptosis, lipid deposition, interferon response, glial cell fate and development. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts as a mRNA modification reader that specifically recognizes and binds mRNA transcripts modified by internal N(7)-methylguanine (m7G). Promotes the formation of circular RNAs (circRNAs) during the epithelial to mesenchymal transition and in cardiomyocytes: acts by binding to sites flanking circRNA-forming exons. CircRNAs are produced by back-splicing circularization of pre-mRNAs. Plays a central role in myelinization via 3 distinct mechanisms (By similarity). First, acts by protecting and promoting stability of target mRNAs such as MBP, SIRT2 and CDKN1B, which promotes oligodendrocyte differentiation. Second, participates in mRNA transport by regulating the nuclear export of MBP mRNA. Finally, indirectly regulates mRNA splicing of MAG pre-mRNA during oligodendrocyte differentiation by acting as a negative regulator of MAG exon 12 alternative splicing: acts by binding to HNRNPA1 mRNA splicing factor, preventing its translation. Involved in microglia differentiation and remyelination by regulating microexon alternative splicing of the Rho GTPase pathway (By similarity). Involved in macrophage differentiation: promotes monocyte differentiation by regulating pre-mRNA splicing in naive peripheral blood monocytes (By similarity). Acts as an important regulator of muscle development: required for the contractile function of cardiomyocytes by regulating alternative splicing of cardiomyocyte transcripts. Acts as a negative regulator of thermogenesis by decreasing stability, nuclear export and translation of mRNAs encoding PPARGC1A and UCP1. Also required for visceral endoderm function and blood vessel development (By similarity). May also play a role in smooth muscle development (By similarity). In addition to its RNA-binding activity, also acts as a nuclear transcription coactivator for SREBF2/SREBP2 (By similarity)
Protein Sequence
10
MVGEMETKEK
20
PKPTPDYLMQ
30
LMNDKKLMSS
40
LPNFCGIFNH
50
LERLLDEEIS
60
RVRKDMYNDT
70
LNGSTEKRSA
80
ELPDAVGPIV
90
QLQEKLYVPV
100
KEYPDFNFVG
110
RILGPRGLTA
120
KQLEAETGCK
130
IMVRGKGSMR
140
DKKKEEQNRG
150
KPNWEHLNED
160
LHVLITVEDA
170
QNRAEIKLKR
180
AVEEVKKLLV
190
PAAEGEDSLK
200
KMQLMELAIL
210
NGTYRDANIK
220
SPALAFSLAA
230
TAQAAPRIIT
240
GPAPVLPPAA
250
LRTPTPAGPT
260
IMPLIRQIQT
270
AVMPNGTPHP
280
TAAIVPPGPE
290
AGLIYTPYEY
300
PYTLAPATSI
310
LEYPIEPSGV
320
LGAVATKVRR
330
HDMRVHPYQR
340
IVTADRAATG
N
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0035198 |
miRNA binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0017124 |
SH3 domain binding |
| Biological Process |
GO:0014004 |
microglia differentiation |
| Biological Process |
GO:0048255 |
mRNA stabilization |
| Biological Process |
GO:0051028 |
mRNA transport |
| Biological Process |
GO:1905869 |
negative regulation of 3'-UTR-mediated mRNA stabilization |
| Biological Process |
GO:0120163 |
negative regulation of cold-induced thermogenesis |
| Biological Process |
GO:0045650 |
negative regulation of macrophage differentiation |
| Biological Process |
GO:2000626 |
negative regulation of miRNA catabolic process |
| Biological Process |
GO:0017148 |
negative regulation of translation |
| Biological Process |
GO:0048710 |
regulation of astrocyte differentiation |
| Biological Process |
GO:0010717 |
regulation of epithelial to mesenchymal transition |
| Biological Process |
GO:0048024 |
regulation of mRNA splicing, via spliceosome |
| Biological Process |
GO:0160091 |
spliceosome-depend formation of circular RNA |
| Biological Process |
GO:0035886 |
vascular associated smooth muscle cell differentiation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.