Overview
| Uniprot ID | Q5XHY0 |
|---|
| Protein Name | ATP-dependent RNA helicase |
|---|
| Gene Name | Ddx18 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 117 |
KKKKKKRKIGNDTEP |
| 58 |
GKAKKALKHSVTAGS |
| 649 |
GGGFGYQKTKKVEKS |
| 660 |
VEKSKIFKHISKKPA |
Function
ATP-dependent RNA helicase that plays a role in the regulation of R-loop homeostasis in both endogenous R-loop-prone regions and at sites of DNA damage. At endogenous loci such as actively transcribed genes, may act as a helicase to resolve the formation of R-loop during transcription and prevent the interference of R-loop with DNA-replication machinery. Also participates in the removal of DNA-lesion-associated R-loop. Plays an essential role for establishing pluripotency during embryogenesis and for pluripotency maintenance in embryonic stem cells. Mechanistically, prevents the polycomb repressive complex 2 (PRC2) from accessing rDNA loci and protects the active chromatin status in nucleolus
Protein Sequence
10
MSQLQMKLLR
20
KKIEKRNAKL
30
RQRNLKLQEA
40
SDRSLSQPEN
50
GDVPEELGKG
60
GKAKKALKHS
70
VTAGSAEAQS
80
RGVPEETVEN
90
AKVKKSPQKL
100
TILTNGEAAA
110
MPSTDPELKK
120
KKKKKRKIGN
130
DTEPDPKKAK
140
TEEGTEAREE
150
PEGAGEEPEG
160
AGEEPEDGVK
170
EADDSEVPSL
180
PLGVTGAFED
190
TSFASLSNLV
200
NENTLKAIEE
210
MGFKHMTEIQ
220
HKSIRPLLEG
230
RDLLAAAKTG
240
SGKTLAFLIP
250
VIELIVKLKF
260
MPRNGTGVLI
270
LSPTRELAMQ
280
TFGVLKELMT
290
HHVHTYGLIM
300
GGSNRSAEVQ
310
KLLNGINIVV
320
ATPGRLLDHM
330
QNTPGFMYKN
340
LQCLVIDEAD
350
RILDVGFEEE
360
LKQIIKLLPA
370
RRQTMLFSAT
380
QTRKVEDLAR
390
ISLKKEPLYV
400
GVDDDKEVAT
410
VDGLEQGYVV
420
CPSEKRFLLL
430
FTFLKKNRKK
440
KVMVFFSSCM
450
SVKYHYELLN
460
YIDLPVLAIH
470
GKQKQNKRTT
480
TFFQFCNADS
490
GILLCTDVAA
500
RGLDIPEVDW
510
IVQYDPPDDP
520
KEYIHRVGRT
530
ARGLNGRGHA
540
LLILRPEELG
550
FLRYLKQSKV
560
PLNQFDFSWS
570
KVSDIQSQLE
580
KLIEKNYFLH
590
KSAQEAYKSY
600
IRAYDSHSLK
610
QIFNVNNLNL
620
PQVALSFGFK
630
VPPFVDLNVS
640
SHDGKLKKRG
650
GGGGFGYQKT
660
KKVEKSKIFK
670
HISKKPADRR
QFSH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0005730 |
nucleolus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016787 |
hydrolase activity |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003724 |
RNA helicase activity |
| Biological Process |
GO:0071392 |
cellular response to estradiol stimulus |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.