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Overview

Uniprot IDQ5XI01
Protein NameLa-related protein 7
Gene NameLarp7
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
245 SSSSGVSKATKRPRT
277 KKKKKRDKVETGGLP
287 TGGLPESKAGKRERS
314 KLKKRAQKDGGGPAA
549 GTEKLITKAEKIRLA
557 AEKIRLAKTQQASQH

Function

RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function. Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II. The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA; on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex. LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing. Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2'-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Binds U6 snRNAs with a 5'-CAGGG-3' sequence motif (By similarity). U6 snRNA processing is required for spermatogenesis (By similarity)

Protein Sequence

10 METENQKTME 20 ESTEKRKEEK 30 KKRSRVKQVL 40 ADIAKQVDFW 50 FGDANLHKDR 60 FLREQIEKSR 70 DGYVDISLLV 80 SFNKMKKLTT 90 DGKLIARALK 100 SSSVVELDLE 110 GTRIRRKKPL 120 GERPKDEEER 130 TVYVELLPKN 140 VTHSWIERVF 150 GKCGNVVYIS 160 IPHYKSTGDP 170 KGFAFVEFET 180 KEQAAKAIEF 190 LNNPPEEAPR 200 KPGIFPKTVK 210 NKPIPSLRVA 220 EEKKKKKKKK 230 GRIKKEESVQ 240 AKELVVDSSS 250 SGVSKATKRP 260 RTASEGSEAE 270 TPEAPKQPAK 280 KKKKRDKVET 290 GGLPESKAGK 300 RERSSAEDED 310 CLPPRPKLKK 320 RAQKDGGGPA 330 ASEVSKEHRD 340 LEFCSTEEEK 350 EPGDRKGDSL 360 SKGKRKHKKK 370 HKERHKMGEE 380 VIPLRVLSKT 390 EWMDLKKEYL 400 ALQKASMASL 410 KKTISQIKLE 420 SEMETESKAP 430 PGSGQQCSTQ 440 EKVSAQGPQF 450 VTGVIVKILS 460 EDPLPGRKQV 470 KDILATISEV 480 VYIDLLEGDT 490 ECHARFKTPE 500 DAQAVMNAQT 510 EIKKKHSWNL 520 EILSGDHEQR 530 YWQKILVDRQ 540 AKLNQPREKK 550 RGTEKLITKA 560 EKIRLAKTQQ 570 ASQHIRFSEY D

Gene Ontology

Classification GO ID Description
Cellular Component GO:0120259 7SK snRNP
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:1990904 ribonucleoprotein complex
Molecular Function GO:0097322 7SK snRNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0017070 U6 snRNA binding
Biological Process GO:0000494 box C/D sno(s)RNA 3'-end processing
Biological Process GO:0030154 cell differentiation
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0036093 germ cell proliferation
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0032897 negative regulation of viral transcription
Biological Process GO:1900087 positive regulation of G1/S transition of mitotic cell cycle
Biological Process GO:1904871 positive regulation of protein localization to Cajal body
Biological Process GO:1905382 positive regulation of snRNA transcription by RNA polymerase II
Biological Process GO:0048024 regulation of mRNA splicing, via spliceosome
Biological Process GO:0008380 RNA splicing
Biological Process GO:0007283 spermatogenesis
Biological Process GO:1990438 U6 2'-O-snRNA methylation

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.