Overview
| Uniprot ID | Q5XI78 |
|---|
| Protein Name | 2-oxoglutarate dehydrogenase complex component E1 |
|---|
| Gene Name | Ogdh |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 577 |
FTRSKDEKILHIKHW |
Function
2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC) (PubMed:18783430). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC (Probable). Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST) (Probable). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids (By similarity). Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate (By similarity). Can also convert 2-keto-4-hydroxyglutarate (KHG) and CoA into malyl-CoA (By similarity). Mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (By similarity)
Protein Sequence
10
MFHLRTCAAK
20
LRPLTASQTV
30
KTFSQNKPAA
40
IRTFQQIRCY
50
SAPVAAEPFL
60
SGTSSNYVEE
70
MYCAWLENPK
80
SVHKSWDIFF
90
RNTNAGAPPG
100
TAYQSPLSLS
110
RSSLATMAHA
120
QSLVEAQPNV
130
DKLVEDHLAV
140
QSLIRAYQIR
150
GHHVAQLDPL
160
GILDADLDSS
170
VPADIISSTD
180
KLGFYGLHES
190
DLDKVFHLPT
200
TTFIGGQEPA
210
LPLREIIRRL
220
EMAYCQHIGV
230
EFMFINDLEQ
240
CQWIRQKFET
250
PGIMQFTNEE
260
KRTLLARLVR
270
STRFEEFLQR
280
KWSSEKRFGL
290
EGCEVLIPAL
300
KTIIDMSSAN
310
GVDYVIMGMP
320
HRGRLNVLAN
330
VIRKELEQIF
340
CQFDSKLEAA
350
DEGSGDMKYH
360
LGMYHRRINR
370
VTDRNITLSL
380
VANPSHLEAA
390
DPVVMGKTKA
400
EQFYCGDTEG
410
KKVMSILLHG
420
DAAFAGQGIV
430
YETFHLSDLP
440
SYTTHGTVHV
450
VVNNQIGFTT
460
DPRMARSSPY
470
PTDVARVVNA
480
PIFHVNSDDP
490
EAVMYVCKVA
500
AEWRNTFHKD
510
VVVDLVCYRR
520
NGHNEMDEPM
530
FTQPLMYKQI
540
RKQKPVLQKY
550
AELLVSQGVV
560
NQPEYEEEIS
570
KYDKICEEAF
580
TRSKDEKILH
590
IKHWLDSPWP
600
GFFTLDGQPR
610
SMTCPSTGLE
620
EDILTHIGNV
630
ASSVPVENFT
640
IHGGLSRILK
650
TRRELVTNRT
660
VDWALAEYMA
670
FGSLLKEGIH
680
VRLSGQDVER
690
GTFSHRHHVL
700
HDQNVDKRTC
710
IPMNHLWPNQ
720
APYTVCNSSL
730
SEYGVLGFEL
740
GFAMASPNAL
750
VLWEAQFGDF
760
NNMAQCIIDQ
770
FICPGQAKWV
780
RQNGIVLLLP
790
HGMEGMGPEH
800
SSARPERFLQ
810
MCNDDPDVLP
820
NLQEENFDIS
830
QLYDCNWIVV
840
NCSTPGNFFH
850
VLRRQILLPF
860
RKPLIVFTPK
870
SLLRHPEART
880
SFDEMLPGTH
890
FQRVIPEDGP
900
AAQNPDKVKR
910
LLFCTGKVYY
920
DLTRERKARD
930
MAEEVAITRI
940
EQLSPFPFDL
950
LLKEAQKYPN
960
AELAWCQEEH
970
KNQGYYDYVK
980
PRLRTTIDRA
990
KPVWYAGRDP
1000
AAAPATGNKK
1010
THLTELQRFL
1020
DTAFDLDAFK
KFS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0031966 |
mitochondrial membrane |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0045252 |
oxoglutarate dehydrogenase complex |
| Molecular Function |
GO:0031072 |
heat shock protein binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0004591 |
oxoglutarate dehydrogenase (succinyl-transferring) activity |
| Molecular Function |
GO:0051087 |
protein-folding chaperone binding |
| Molecular Function |
GO:0030976 |
thiamine pyrophosphate binding |
| Biological Process |
GO:0120551 |
2-oxoglutarate decarboxylation to succinyl-CoA |
| Biological Process |
GO:0006103 |
2-oxoglutarate metabolic process |
| Biological Process |
GO:0021695 |
cerebellar cortex development |
| Biological Process |
GO:0006091 |
generation of precursor metabolites and energy |
| Biological Process |
GO:0006096 |
glycolytic process |
| Biological Process |
GO:0021766 |
hippocampus development |
| Biological Process |
GO:0061034 |
olfactory bulb mitral cell layer development |
| Biological Process |
GO:0021860 |
pyramidal neuron development |
| Biological Process |
GO:0021756 |
striatum development |
| Biological Process |
GO:0006104 |
succinyl-CoA metabolic process |
| Biological Process |
GO:0022028 |
tangential migration from the subventricular zone to the olfactory bulb |
| Biological Process |
GO:0021794 |
thalamus development |
| Biological Process |
GO:0006099 |
tricarboxylic acid cycle |
Reference
[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.