Overview
| Uniprot ID | Q5XIC8 |
|---|
| Protein Name | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 |
|---|
| Gene Name | Alkbh3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 19 |
GAWATPTKSQSAARP |
| 57 |
CDRRFVFKEPQLVVR |
Function
Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron
Protein Sequence
10
MGDKRQRARV
20
QGAWATPTKS
30
QSAARPATPA
40
RSRPSQTPGP
50
SWRSKEQQQC
60
DRRFVFKEPQ
70
LVVRAAPEPR
80
VIDREGVYEI
90
SLSPTGVSRV
100
CLYPGFVDLK
110
EADWILERLC
120
QDVPWKQRMG
130
IREDITYPQP
140
RLTAWYGELP
150
YTYSRVTMEP
160
NPHWLPVLWT
170
LKSRIEENTG
180
HTFNSLLCNF
190
YRDEKDSVDW
200
HSDDEPSLGS
210
CPVIASLSFG
220
ATRTFEMRKK
230
PPPEENGDYT
240
YVERVKIPLD
250
HGTLLIMEGA
260
TQADWQHRVP
270
KEYHSRERRV
280
NLTFRTVYPD
290
PRGAPGDTSA
ELPLR
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Molecular Function |
GO:0016706 |
2-oxoglutarate-dependent dioxygenase activity |
| Molecular Function |
GO:0035516 |
broad specificity oxidative DNA demethylase activity |
| Molecular Function |
GO:0051747 |
cytosine C-5 DNA demethylase activity |
| Molecular Function |
GO:0008198 |
ferrous iron binding |
| Molecular Function |
GO:1990930 |
mRNA N1-methyladenosine dioxygenase activity |
| Molecular Function |
GO:0035515 |
oxidative RNA demethylase activity |
| Biological Process |
GO:0008283 |
cell population proliferation |
| Biological Process |
GO:0006307 |
DNA alkylation repair |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:2000766 |
negative regulation of cytoplasmic translation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.