Overview
| Uniprot ID | Q5XIP6 |
|---|
| Protein Name | Flap endonuclease 1 |
|---|
| Gene Name | Fen1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 345 |
GRLDDFFKVTGSLSS |
| 375 |
AKTGGAGKFRRGK** |
Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA
Protein Sequence
10
MGIQGLAKLI
20
ADVAPSAIRE
30
NDIKSYFGRK
40
VAIDASMSIY
50
QFLIAVRQGG
60
DVLQNEEGET
70
TSHLMGMFYR
80
TIRMMENGIK
90
PVYIFDGKPP
100
QLKSGELAKR
110
SERRAEAEKQ
120
LQQAQEAGAE
130
EEVEKFTKRL
140
VKVTKQHNDE
150
CKHLLSLMGI
160
PYLDAPSEAE
170
ASCAALAKAG
180
KVYAAATEDM
190
DCLTFGSPVL
200
MRHLTASEAK
210
KLPIQEFHLS
220
RVLQELGLNQ
230
EQFVDLCILL
240
GSDYCESVRG
250
IGPKRAVDLI
260
QKHKSIEEIV
270
RRLDPSKYPV
280
PENWLHKEAR
290
QLFLEPEVLD
300
PESVELKWSE
310
PNEEELVKFM
320
CGEKQFSEER
330
IRSGVKRLNK
340
SRQGSTQGRL
350
DDFFKVTGSL
360
SSAKRKEPEP
370
KGPAKKKAKT
380
GGAGKFRRGK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0008409 |
5'-3' exonuclease activity |
| Molecular Function |
GO:0017108 |
5'-flap endonuclease activity |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0004527 |
exonuclease activity |
| Molecular Function |
GO:0048256 |
flap endonuclease activity |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Molecular Function |
GO:0030145 |
manganese ion binding |
| Molecular Function |
GO:0004523 |
RNA-DNA hybrid ribonuclease activity |
| Biological Process |
GO:0006284 |
base-excision repair |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0043137 |
DNA replication, removal of RNA primer |
| Biological Process |
GO:0007613 |
memory |
| Biological Process |
GO:0045876 |
positive regulation of sister chromatid cohesion |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.