Overview
| Uniprot ID | Q5XIX3 |
|---|
| Protein Name | Protein artemis |
|---|
| Gene Name | Dclre1c |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 279 |
CGMASKTKTVLHTIS |
Function
Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation, and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ (By similarity)
Protein Sequence
10
MSSFQGQMEE
20
YPTISIDRFD
30
RENLKARAYF
40
LSHCHKDHMK
50
GLRAPSMKRR
60
LECSLKVFLY
70
CSPVTKELLL
80
TSPKYKFWEN
90
RIIAIEIETP
100
TQVSLVDEAS
110
GEKEEVVVTL
120
LPAGHCPGSV
130
MFLFQGSNGT
140
VLYTGDFRLA
150
KGEVSRMELL
160
HSGGRVKDIQ
170
SVYLDTTFCD
180
PRFYQIPSRE
190
ECLRGVLELV
200
RSWITRSPKH
210
VVWLNCKAAY
220
GYEYLFTNLS
230
EELGVQVHVD
240
KLDMFKNMPD
250
ILHHLTTDRN
260
TQIHACRHPK
270
AEEYFQWNKL
280
PCGMASKTKT
290
VLHTISIKPS
300
TMWFGERTRK
310
TNVIVRTGES
320
SYRACFSFHS
330
SYSEIKDFLS
340
YICPVNAYPN
350
VIPIGLTVDK
360
VMDFLKPLCR
370
SSQCAEPKYK
380
PLGKLKRART
390
VHLDSEEDDD
400
LFDDPLLTHS
410
RRKVPYQVTL
420
HPEVFSMKAL
430
PLDQPELGQS
440
PGCCKAESMP
450
SPSLANFVDC
460
DESNSDSEGE
470
LETPPSLQGG
480
LGPTTLPQQN
490
ADPDVDVPRW
500
EVFFKRKDEI
510
TDECLENLPS
520
SIETGGSQSP
530
KRFSDSPKLG
540
SDSDGESTHI
550
SSQNSSQSTH
560
ITDQGSQGWD
570
SQCDTVLLSS
580
QEKSGGDSTS
590
LNKDTYKPKP
600
KDSISASQIE
610
QNALCPQDTH
620
CDLKSGAEVN
630
GVPCIEEPDT
640
VSGRKSSPEK
650
TSLTSTQADS
660
QSSSDFEIPS
670
TPEAELPKPE
680
HLQFLYGKLA
690
TGESIVLKKE
NVHSQIFK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0070419 |
nonhomologous end joining complex |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0035312 |
5'-3' DNA exonuclease activity |
| Molecular Function |
GO:0008409 |
5'-3' exonuclease activity |
| Molecular Function |
GO:0003684 |
damaged DNA binding |
| Molecular Function |
GO:0000014 |
single-stranded DNA endodeoxyribonuclease activity |
| Biological Process |
GO:0002250 |
adaptive immune response |
| Biological Process |
GO:0030183 |
B cell differentiation |
| Biological Process |
GO:0051276 |
chromosome organization |
| Biological Process |
GO:0006310 |
DNA recombination |
| Biological Process |
GO:0006302 |
double-strand break repair |
| Biological Process |
GO:0006303 |
double-strand break repair via nonhomologous end joining |
| Biological Process |
GO:0036297 |
interstrand cross-link repair |
| Biological Process |
GO:0010212 |
response to ionizing radiation |
| Biological Process |
GO:0000723 |
telomere maintenance |
| Biological Process |
GO:0033151 |
V(D)J recombination |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.