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Overview

Uniprot IDQ60749
Protein NameKH domain-containing, RNA-binding, signal transduction-associated protein 1
Gene NameKhdrbs1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
21 SSGRSCSKDPSGAHP
424 NGTRPSLKAPPARPV

Function

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. May not be involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (By similarity). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner. Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (PubMed:12478298, PubMed:22196734, PubMed:24469635)

Protein Sequence

10 MQRRDDPASR 20 LTRSSGRSCS 30 KDPSGAHPSV 40 RLTPSRPSPL 50 PHRPRGGGGG 60 PRGGARASPA 70 TQPPPLLPPS 80 TPGPDATVVG 90 SAPTPLLPPS 100 ATAAVKMEPE 110 NKYLPELMAE 120 KDSLDPSFTH 130 AMQLLSVEIE 140 KIQKGESKKD 150 DEENYLDLFS 160 HKNMKLKERV 170 LIPVKQYPKF 180 NFVGKILGPQ 190 GNTIKRLQEE 200 TGAKISVLGK 210 GSMRDKAKEE 220 ELRKGGDPKY 230 AHLNMDLHVF 240 IEVFGPPCEA 250 YALMAHAMEE 260 VKKFLVPDMM 270 DDICQEQFLE 280 LSYLNGVPEP 290 SRGRGVSVRG 300 RGAAPPPPPV 310 PRGRGVGPPR 320 GALVRGTPVR 330 GSITRGATVT 340 RGVPPPPTVR 350 GAPTPRARTA 360 GIQRIPLPPT 370 PAPETYEDYG 380 YDDTYAEQSY 390 EGYEGYYSQS 400 QGESEYYDYG 410 HGELQDSYEA 420 YGQDDWNGTR 430 PSLKAPPARP 440 VKGAYREHPY GRY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0070618 Grb2-Sos complex
Cellular Component GO:0016020 membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0140678 molecular function inhibitor activity
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0008143 poly(A) binding
Molecular Function GO:0008266 poly(U) RNA binding
Molecular Function GO:0019904 protein domain specific binding
Molecular Function GO:1990782 protein tyrosine kinase binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0042169 SH2 domain binding
Molecular Function GO:0017124 SH3 domain binding
Molecular Function GO:0035591 signaling adaptor activity
Biological Process GO:0007166 cell surface receptor signaling pathway
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0046833 positive regulation of RNA export from nucleus
Biological Process GO:0045948 positive regulation of translational initiation
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:0042981 regulation of apoptotic process
Biological Process GO:0051726 regulation of cell cycle
Biological Process GO:0048024 regulation of mRNA splicing, via spliceosome
Biological Process GO:0046831 regulation of RNA export from nucleus
Biological Process GO:0043484 regulation of RNA splicing
Biological Process GO:0007283 spermatogenesis
Biological Process GO:0050852 T cell receptor signaling pathway

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.