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Overview

Uniprot IDQ60930
Protein NameNon-selective voltage-gated ion channel VDAC2
Gene NameVdac2
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
121 TFSPNTGKKSGKIKS
73 VSGTLETKYKWCEYG
75 GTLETKYKWCEYGLT

Function

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:10430654). The channel adopts an open conformation at zero mV and a closed conformation at both positive and negative potentials (By similarity). There are two populations of channels; the main that functions in a lower open-state conductance with lower ion selectivity, that switch, in a voltage-dependent manner, from the open to a low-conducting 'closed' state and the other that has a normal ion selectivity in the typical high conductance, 'open' state (PubMed:10430654). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:34799735). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (By similarity). Associates with the translocase of the outer mitochondrial membrane (TOM) complex and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (By similarity)

Protein Sequence

10 MAECCVPVCP 20 RPMCIPPPYA 30 DLGKAARDIF 40 NKGFGFGLVK 50 LDVKTKSCSG 60 VEFSTSGSSN 70 TDTGKVSGTL 80 ETKYKWCEYG 90 LTFTEKWNTD 100 NTLGTEIAIE 110 DQICQGLKLT 120 FDTTFSPNTG 130 KKSGKIKSAY 140 KRECINLGCD 150 VDFDFAGPAI 160 HGSAVFGYEG 170 WLAGYQMTFD 180 SAKSKLTRSN 190 FAVGYRTGDF 200 QLHTNVNNGT 210 EFGGSIYQKV 220 CEDFDTSVNL 230 AWTSGTNCTR 240 FGIAAKYQLD 250 PTASISAKVN 260 NSSLIGVGYT 270 QTLRPGVKLT 280 LSALVDGKSF 290 NAGGHKLGLA LELEA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0043209 myelin sheath
Cellular Component GO:0046930 pore complex
Cellular Component GO:0097225 sperm midpiece
Cellular Component GO:0008021 synaptic vesicle
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0097001 ceramide binding
Molecular Function GO:0015485 cholesterol binding
Molecular Function GO:0008142 oxysterol binding
Molecular Function GO:0031210 phosphatidylcholine binding
Molecular Function GO:0017128 phospholipid scramblase activity
Molecular Function GO:0015288 porin activity
Molecular Function GO:0008308 voltage-gated monoatomic anion channel activity
Molecular Function GO:0005244 voltage-gated monoatomic ion channel activity
Biological Process GO:0007339 binding of sperm to zona pellucida
Biological Process GO:0097345 mitochondrial outer membrane permeabilization
Biological Process GO:1990542 mitochondrial transmembrane transport
Biological Process GO:0006820 monoatomic anion transport
Biological Process GO:2001243 negative regulation of intrinsic apoptotic signaling pathway
Biological Process GO:0032272 negative regulation of protein polymerization
Biological Process GO:0045332 phospholipid translocation
Cellular Component GO:0001669 acrosomal vesicle
Cellular Component GO:0016020 membrane
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0042645 mitochondrial nucleoid

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.