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Overview

Uniprot IDQ60932
Protein NameNon-selective voltage-gated ion channel VDAC1
Gene NameVdac1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
122 SFSPNTGKKNAKIKT
279 LSALLDGKNVNAGGH
33 SARDVFTKGYGFGLI

Function

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:34799735). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:10716730, PubMed:15477379, PubMed:18988731). May mediate ATP export from cells (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (PubMed:29907098)

Protein Sequence

10 MCSFFLVLLL 20 WQNMAVPPTY 30 ADLGKSARDV 40 FTKGYGFGLI 50 KLDLKTKSEN 60 GLEFTSSGSA 70 NTETTKVNGS 80 LETKYRWTEY 90 GLTFTEKWNT 100 DNTLGTEITV 110 EDQLARGLKL 120 TFDSSFSPNT 130 GKKNAKIKTG 140 YKREHINLGC 150 DVDFDIAGPS 160 IRGALVLGYE 170 GWLAGYQMNF 180 ETSKSRVTQS 190 NFAVGYKTDE 200 FQLHTNVNDG 210 TEFGGSIYQK 220 VNKKLETAVN 230 LAWTAGNSNT 240 RFGIAAKYQV 250 DPDACFSAKV 260 NNSSLIGLGY 270 TQTLKPGIKL 280 TLSALLDGKN 290 VNAGGHKLGL GLEFQA

Gene Ontology

Classification GO ID Description
Biological Process GO:0006820 monoatomic anion transport
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0110099 negative regulation of calcium import into the mitochondrion
Biological Process GO:2000378 negative regulation of reactive oxygen species metabolic process
Biological Process GO:0007270 neuron-neuron synaptic transmission
Biological Process GO:0043065 positive regulation of apoptotic process
Biological Process GO:1901526 positive regulation of mitophagy
Biological Process GO:1905091 positive regulation of type 2 mitophagy
Biological Process GO:0006086 pyruvate decarboxylation to acetyl-CoA
Biological Process GO:1901524 regulation of mitophagy
Cellular Component GO:0016020 membrane
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0042645 mitochondrial nucleoid
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0005757 mitochondrial permeability transition pore complex
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0043209 myelin sheath
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0098839 postsynaptic density membrane
Cellular Component GO:0048787 presynaptic active zone membrane
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0008021 synaptic vesicle
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0097001 ceramide binding
Molecular Function GO:0015485 cholesterol binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0005253 monoatomic anion channel activity
Molecular Function GO:0008142 oxysterol binding
Molecular Function GO:0031210 phosphatidylcholine binding
Molecular Function GO:0015288 porin activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0044325 transmembrane transporter binding
Molecular Function GO:0022832 voltage-gated channel activity
Molecular Function GO:0008308 voltage-gated monoatomic anion channel activity
Molecular Function GO:0005244 voltage-gated monoatomic ion channel activity
Biological Process GO:0006915 apoptotic process
Biological Process GO:0001662 behavioral fear response
Biological Process GO:0036444 calcium import into the mitochondrion
Biological Process GO:0007268 chemical synaptic transmission
Biological Process GO:0030855 epithelial cell differentiation
Biological Process GO:0007612 learning
Biological Process GO:0006869 lipid transport
Biological Process GO:0006851 mitochondrial calcium ion transmembrane transport
Biological Process GO:1990542 mitochondrial transmembrane transport

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.