Overview
| Uniprot ID | Q61166 |
|---|
| Protein Name | Microtubule-associated protein RP/EB family member 1 |
|---|
| Gene Name | Mapre1 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 151 |
PALSKPKKPLGSSTA |
| 174 |
QRTTAAPKAGPGMVR |
Function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains. Promotes cytoplasmic microtubule nucleation and elongation. Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation. Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (By similarity). Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation (PubMed:15311282). Promotes microtubule-mediated GJA1/CX43 trafficking to cell membranes via its interaction with DSP, thereby facilitating gap junction intracellular communication (By similarity). May play a role in cell migration (PubMed:15311282)
Protein Sequence
10
MAVNVYSTSV
20
TSDNLSRHDM
30
LAWINESLQL
40
NLTKIEQLCS
50
GAAYCQFMDM
60
LFPGSIALKK
70
VKFQAKLEHE
80
YIQNFKILQA
90
GFKRMGVDKI
100
IPVDKLVKGK
110
FQDNFEFVQW
120
FKKFFDANYD
130
GKEYDPVAAR
140
QGQETAVAPS
150
LVAPALSKPK
160
KPLGSSTAAP
170
QRPIATQRTT
180
AAPKAGPGMV
190
RKNPGVGNGD
200
DEAAELMQQV
210
KVLKLTVEDL
220
EKERDFYFGK
230
LRNIELICQE
240
NEGENDPVLQ
250
RIVDILYATD
260
EGFVIPDEGG
PQEEQEEY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0042995 |
cell projection |
| Cellular Component |
GO:0031253 |
cell projection membrane |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0036064 |
ciliary basal body |
| Cellular Component |
GO:0030981 |
cortical microtubule cytoskeleton |
| Cellular Component |
GO:0005881 |
cytoplasmic microtubule |
| Cellular Component |
GO:0005925 |
focal adhesion |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0005874 |
microtubule |
| Cellular Component |
GO:0015630 |
microtubule cytoskeleton |
| Cellular Component |
GO:0005815 |
microtubule organizing center |
| Cellular Component |
GO:0035371 |
microtubule plus-end |
| Cellular Component |
GO:1905721 |
mitotic spindle astral microtubule end |
| Cellular Component |
GO:1990498 |
mitotic spindle microtubule |
| Cellular Component |
GO:0097431 |
mitotic spindle pole |
| Cellular Component |
GO:0051233 |
spindle midzone |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0051010 |
microtubule plus-end binding |
| Molecular Function |
GO:0120283 |
protein serine/threonine kinase binding |
| Biological Process |
GO:0051315 |
attachment of mitotic spindle microtubules to kinetochore |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0016477 |
cell migration |
| Biological Process |
GO:0000132 |
establishment of mitotic spindle orientation |
| Biological Process |
GO:0001578 |
microtubule bundle formation |
| Biological Process |
GO:0046785 |
microtubule polymerization |
| Biological Process |
GO:0031115 |
negative regulation of microtubule polymerization |
| Biological Process |
GO:1905515 |
non-motile cilium assembly |
| Biological Process |
GO:0031116 |
positive regulation of microtubule polymerization |
| Biological Process |
GO:1902888 |
protein localization to astral microtubule |
| Biological Process |
GO:0071539 |
protein localization to centrosome |
| Biological Process |
GO:0035372 |
protein localization to microtubule |
| Biological Process |
GO:1902480 |
protein localization to mitotic spindle |
| Biological Process |
GO:0031110 |
regulation of microtubule polymerization or depolymerization |
| Biological Process |
GO:0051225 |
spindle assembly |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.