Search Results

Overview

Uniprot IDQ61425
Protein NameHydroxyacyl-coenzyme A dehydrogenase, mitochondrial
Gene NameHadh
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
127 IVENLKLKNELFQRL
185 MKLVEVIKTPMTSQK
206 DFCKTLGKHPVSCKD
212 GKHPVSCKDTPGFIV
241 HERGDASKEDIDTAM
305 AQKKLGKKTGEGFYK
312 KTGEGFYKYK*****
68 DILAKSKKGIEESLK
75 KGIEESLKRMAKKKF
81 LKRMAKKKFTENPKA
87 KKFTENPKAGDEFVE

Function

Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (By similarity). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (PubMed:20670938). Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes (PubMed:36398584)

Protein Sequence

10 MAFVTRQFLR 20 SMSSSSSASA 30 AAKKILIKHV 40 TVIGGGLMGA 50 GIAQVAAATG 60 HTVVLVDQTE 70 DILAKSKKGI 80 EESLKRMAKK 90 KFTENPKAGD 100 EFVEKTLSCL 110 STSTDAASVV 120 HSTDLVVEAI 130 VENLKLKNEL 140 FQRLDKFAAE 150 HTIFASNTSS 160 LQITNIANAT 170 TRQDRFAGLH 180 FFNPVPMMKL 190 VEVIKTPMTS 200 QKTFESLVDF 210 CKTLGKHPVS 220 CKDTPGFIVN 230 RLLVPYLIEA 240 VRLHERGDAS 250 KEDIDTAMKL 260 GAGYPMGPFE 270 LLDYVGLDTT 280 KFILDGWHEM 290 EPENPLFQPS 300 PSMNNLVAQK 310 KLGKKTGEGF YKYK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0003857 (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0070403 NAD+ binding
Biological Process GO:0030154 cell differentiation
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0046676 negative regulation of insulin secretion
Biological Process GO:0120162 positive regulation of cold-induced thermogenesis
Biological Process GO:0050796 regulation of insulin secretion
Biological Process GO:0014823 response to activity
Biological Process GO:0032868 response to insulin
Biological Process GO:0009410 response to xenobiotic stimulus
Biological Process GO:0007283 spermatogenesis

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.