Search Results

Overview

Uniprot ID	Q61656
Protein Name	Probable ATP-dependent RNA helicase DDX5
Gene Name	Ddx5
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
33	TGPLSGKKFGNPGEK

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms

Protein Sequence

10 MSSYSSDRDR 20 GRDRGFGAPR 30 FGGSRTGPLS 40 GKKFGNPGEK 50 LVKKKWNLDE 60 LPKFEKNFYQ 70 EHPDLARRTA 80 QEVDTYRRSK 90 EITVRGHNCP 100 KPVLNFYEAN 110 FPANVMDVIA 120 RQNFTEPTAI 130 QAQGWPVALS 140 GLDMVGVAQT 150 GSGKTLSYLL 160 PAIVHINHQP 170 FLERGDGPIC 180 LVLAPTRELA 190 QQVQQVAAEY 200 CRACRLKSTC 210 IYGGAPKGPQ 220 IRDLERGVEI 230 CIATPGRLID 240 FLECGKTNLR 250 RTTYLVLDEA 260 DRMLDMGFEP 270 QIRKIVDQIR 280 PDRQTLMWSA 290 TWPKEVRQLA 300 EDFLKDYIHI 310 NIGALELSAN 320 HNILQIVDVC 330 HDVEKDEKLI 340 RLMEEIMSEK 350 ENKTIVFVET 360 KRRCDELTRK 370 MRRDGWPAMG 380 IHGDKSQQER 390 DWVLNEFKHG 400 KAPILIATDV 410 ASRGLDVEDV 420 KFVINYDYPN 430 SSEDYIHRIG 440 RTARSTKTGT 450 AYTFFTPNNI 460 KQVSDLISVL 470 REANQAINPK 480 LLQLVEDRGS 490 GRSRGRGGMK 500 DDRRDRYSAG 510 KRGGFNTFRD 520 RENYDRGYSN 530 LLKRDFGAKT 540 QNGVYSAANY 550 TNGSFGSNFV 560 SAGIQTSFRT 570 GNPTGTYQNG 580 YDSTQQYGSN 590 VANMHNGMNQ 600 QAYAYPLPQA 610 APMIGYPMPT GYSQ

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0071013	catalytic step 2 spliceosome
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:1990904	ribonucleoprotein complex
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0048306	calcium-dependent protein binding
Molecular Function	GO:0005516	calmodulin binding
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0035500	MH2 domain binding
Molecular Function	GO:0003730	mRNA 3'-UTR binding
Molecular Function	GO:0003729	mRNA binding
Molecular Function	GO:0050681	nuclear androgen receptor binding
Molecular Function	GO:0036002	pre-mRNA binding
Molecular Function	GO:0070878	primary miRNA binding
Molecular Function	GO:1990841	promoter-specific chromatin binding
Molecular Function	GO:0070412	R-SMAD binding
Molecular Function	GO:0043021	ribonucleoprotein complex binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003724	RNA helicase activity
Molecular Function	GO:0046332	SMAD binding
Molecular Function	GO:0003712	transcription coregulator activity
Biological Process	GO:0000380	alternative mRNA splicing, via spliceosome
Biological Process	GO:0030521	androgen receptor signaling pathway
Biological Process	GO:0030509	BMP signaling pathway
Biological Process	GO:0007623	circadian rhythm
Biological Process	GO:0001837	epithelial to mesenchymal transition
Biological Process	GO:0030520	estrogen receptor signaling pathway
Biological Process	GO:0072332	intrinsic apoptotic signaling pathway by p53 class mediator
Biological Process	GO:0061614	miRNA transcription
Biological Process	GO:0009299	mRNA transcription
Biological Process	GO:0045445	myoblast differentiation
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:0000956	nuclear-transcribed mRNA catabolic process
Biological Process	GO:0043517	positive regulation of DNA damage response, signal transduction by p53 class mediator
Biological Process	GO:0045893	positive regulation of DNA-templated transcription
Biological Process	GO:0031053	primary miRNA processing
Biological Process	GO:0006606	protein import into nucleus
Biological Process	GO:0000381	regulation of alternative mRNA splicing, via spliceosome
Biological Process	GO:0060765	regulation of androgen receptor signaling pathway
Biological Process	GO:0045667	regulation of osteoblast differentiation
Biological Process	GO:2001014	regulation of skeletal muscle cell differentiation
Biological Process	GO:0006357	regulation of transcription by RNA polymerase II
Biological Process	GO:0045069	regulation of viral genome replication

Reference

[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.