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Overview

Uniprot ID	Q62425
Protein Name	Cytochrome c oxidase subunit FA4
Gene Name	Coxfa4
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
10	RQILGQAKKHPSLIP
74	SVNVDYSKLKKEGPD

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. COXFA4 is required for complex IV maintenance

Protein Sequence

10 MLRQILGQAK 20 KHPSLIPLFV 30 FIGAGGTGAA 40 LYVMRLALFN 50 PDVSWDRKNN 60 PEPWNKLGPN 70 EQYKFYSVNV 80 DYSKLKKEGP DF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0045271	respiratory chain complex I
Cellular Component	GO:0045277	respiratory chain complex IV
Molecular Function	GO:0044877	protein-containing complex binding
Biological Process	GO:0022904	respiratory electron transport chain

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.