Overview
| Uniprot ID | Q62634 |
|---|
| Protein Name | Vesicular glutamate transporter 1 |
|---|
| Gene Name | Slc17a7 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 272 |
SISEEERKYIEDAIG |
Function
Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed:10938000, PubMed:11001057, PubMed:11698619, PubMed:18080752, PubMed:19169251, PubMed:25433636, PubMed:27133463, PubMed:29642010, PubMed:33440152, PubMed:8202535). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:10938000, PubMed:11001057, PubMed:11698619, PubMed:18080752, PubMed:29642010). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed:29642010). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (PubMed:10938000, PubMed:19169251, PubMed:25433636, PubMed:27133463, PubMed:29642010). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (PubMed:25433636). The vesicular K(+)/H(+) antiport activity is electroneutral (PubMed:25433636). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed:29642010, PubMed:33440152, PubMed:8202535). The symporter activity is driven by an inside negative membrane potential and is electrogenic (PubMed:29642010). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity)
Protein Sequence
10
MEFRQEEFRK
20
LAGRALGRLH
30
RLLEKRQEGA
40
ETLELSADGR
50
PVTTHTRDPP
60
VVDCTCFGLP
70
RRYIIAIMSG
80
LGFCISFGIR
90
CNLGVAIVSM
100
VNNSTTHRGG
110
HVVVQKAQFN
120
WDPETVGLIH
130
GSFFWGYIVT
140
QIPGGFICQK
150
FAANRVFGFA
160
IVATSTLNML
170
IPSAARVHYG
180
CVIFVRILQG
190
LVEGVTYPAC
200
HGIWSKWAPP
210
LERSRLATTA
220
FCGSYAGAVV
230
AMPLAGVLVQ
240
YSGWSSVFYV
250
YGSFGIFWYL
260
FWLLVSYESP
270
ALHPSISEEE
280
RKYIEDAIGE
290
SAKLMNPVTK
300
FNTPWRRFFT
310
SMPVYAIIVA
320
NFCRSWTFYL
330
LLISQPAYFE
340
EVFGFEISKV
350
GLVSALPHLV
360
MTIIVPIGGQ
370
IADFLRSRHI
380
MSTTNVRKLM
390
NCGGFGMEAT
400
LLLVVGYSHS
410
KGVAISFLVL
420
AVGFSGFAIS
430
GFNVNHLDIA
440
PRYASILMGI
450
SNGVGTLSGM
460
VCPIIVGAMT
470
KHKTREEWQY
480
VFLIASLVHY
490
GGVIFYGVFA
500
SGEKQPWAEP
510
EEMSEEKCGF
520
VGHDQLAGSD
530
ESEMEDEVEP
540
PGAPPAPPPS
550
YGATHSTVQP
560
PRPPPPVRDY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0044300 |
cerebellar mossy fiber |
| Cellular Component |
GO:0034707 |
chloride channel complex |
| Cellular Component |
GO:0060076 |
excitatory synapse |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0048786 |
presynaptic active zone |
| Cellular Component |
GO:0045202 |
synapse |
| Cellular Component |
GO:0008021 |
synaptic vesicle |
| Cellular Component |
GO:0030672 |
synaptic vesicle membrane |
| Molecular Function |
GO:0005254 |
chloride channel activity |
| Molecular Function |
GO:0008068 |
extracellularly glutamate-gated chloride channel activity |
| Molecular Function |
GO:0005313 |
L-glutamate transmembrane transporter activity |
| Molecular Function |
GO:0140788 |
L-glutamate uniporter activity |
| Molecular Function |
GO:0005326 |
neurotransmitter transmembrane transporter activity |
| Molecular Function |
GO:0140787 |
phosphate ion uniporter activity |
| Molecular Function |
GO:0015386 |
potassium:proton antiporter activity |
| Molecular Function |
GO:0005436 |
sodium:phosphate symporter activity |
| Biological Process |
GO:0007268 |
chemical synaptic transmission |
| Biological Process |
GO:0006821 |
chloride transport |
| Biological Process |
GO:0060079 |
excitatory postsynaptic potential |
| Biological Process |
GO:0051938 |
L-glutamate import |
| Biological Process |
GO:0015813 |
L-glutamate transmembrane transport |
| Biological Process |
GO:0007616 |
long-term memory |
| Biological Process |
GO:0003407 |
neural retina development |
| Biological Process |
GO:0098700 |
neurotransmitter loading into synaptic vesicle |
| Biological Process |
GO:0001504 |
neurotransmitter uptake |
| Biological Process |
GO:0055062 |
phosphate ion homeostasis |
| Biological Process |
GO:0035435 |
phosphate ion transmembrane transport |
| Biological Process |
GO:0006817 |
phosphate ion transport |
| Biological Process |
GO:0006813 |
potassium ion transport |
| Biological Process |
GO:0050803 |
regulation of synapse structure or activity |
| Biological Process |
GO:1900242 |
regulation of synaptic vesicle endocytosis |
| Biological Process |
GO:0044341 |
sodium-dependent phosphate transport |
| Biological Process |
GO:0035249 |
synaptic transmission, glutamatergic |
| Biological Process |
GO:0097401 |
synaptic vesicle lumen acidification |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.