Overview
| Uniprot ID | Q62651 |
|---|
| Protein Name | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial |
|---|
| Gene Name | Ech1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 150 |
DLISRYQKTFTVIEK |
| 230 |
ELTFTARKMMADEAL |
| 76 |
VQLNRPEKRNAMNRA |
Function
Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids. Together with the 2,4-dienoyl-CoA reductase (DECR) and the Enoyl-CoA delta isomerase 1 (ECI1) they allow reentrance of the enoyl-CoA into the beta-oxidation cycle (PubMed:11278886, PubMed:9417087). Able to isomerize 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position (PubMed:11278886, PubMed:9417087). Based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (By similarity)
Protein Sequence
10
MATAMTVSSK
20
LLGLLMQQLR
30
GTRQLYFNVS
40
LRSLSSSAQE
50
ASKRIPEEVS
60
DHNYESIQVT
70
SAQKHVLHVQ
80
LNRPEKRNAM
90
NRAFWRELVE
100
CFQKISKDSD
110
CRAVVVSGAG
120
KMFTSGIDLM
130
DMASDILQPP
140
GDDVARIAWY
150
LRDLISRYQK
160
TFTVIEKCPK
170
PVIAAIHGGC
180
IGGGVDLISA
190
CDIRYCTQDA
200
FFQVKEVDVG
210
LAADVGTLQR
220
LPKVIGNRSL
230
VNELTFTARK
240
MMADEALDSG
250
LVSRVFPDKD
260
VMLNAAFALA
270
ADISSKSPVA
280
VQGSKINLIY
290
SRDHSVDESL
300
DYMATWNMSM
310
LQTQDIIKSV
320
QAAMEKKDSK
SITFSKL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005777 |
peroxisome |
| Molecular Function |
GO:0051750 |
delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity |
| Biological Process |
GO:0006635 |
fatty acid beta-oxidation |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.