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Overview

Uniprot IDQ62780
Protein NameProbable ATP-dependent RNA helicase DDX46
Gene NameDdx46
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
1026 NSYQPTNKGRYKVL*
263 KSGPTVTKVVTVVTT
271 VVTVVTTKKAVVDAD
272 VTVVTTKKAVVDADK
779 GFSGKGFKFDETEQA

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, DDX46 plays essential roles during assembly of pre-spliceosome and proofreading of the branch site

Protein Sequence

10 MGRESRHYRK 20 RSASRGRSGS 30 RSRSRSPSDK 40 RSKRGDDRRS 50 RSRDRDRRRE 60 RSRSRDKRRS 70 RSRDRKRLRR 80 SRSRERDRSR 90 ERRRSRSRDR 100 RRSRSRSRGR 110 RSRSSSPGSK 120 SKKAENRSRS 130 KEKAEGGDSS 140 KEKKKDKDDK 150 EDEKEKDAGN 160 FDQNKLEEEM 170 RKRKERVEKW 180 REEQRKKAME 190 NIGELKKEIE 200 EMKQGKKWSL 210 EDDDDDEDDP 220 AEAEKEGNEM 230 EDEELDPLDA 240 YMEEVKEEVK 250 KFNMRSVKGG 260 AGNEKKSGPT 270 VTKVVTVVTT 280 KKAVVDADKK 290 KGELMENDQD 300 AMEYSSEEEE 310 VDLQTALTGY 320 QTKQRKLLEP 330 VDHGKIEYEP 340 FRKNFYVEVP 350 ELAKMSQEEV 360 NVFRLEMEGI 370 TVKGKGCPKP 380 IKSWVQCGIS 390 MKILNSLKKH 400 GYEKPTPIQT 410 QAIPAIMSGR 420 DLIGIAKTGS 430 GKTIAFLLPM 440 FRHIMDQRSL 450 EEGEGPIAVI 460 MTPTRELALQ 470 ITKECKKFSK 480 TLGLRVVCVY 490 GGTGISEQIA 500 ELKRGAEIIV 510 CTPGRMIDML 520 AANSGRVTNL 530 RRVTYVVLDE 540 ADRMFDMGFE 550 PQVMRIVDNV 560 RPDRQTVMFS 570 ATFPRAMEAL 580 ARRILSKPIE 590 VQVGGRSVVC 600 SDVEQQVIVI 610 EEEKKFLKLL 620 ELLGHYQESG 630 SVIIFVDKQE 640 HADGLLKDLM 650 RASYPCMSLH 660 GGIDQYDRDS 670 IINDFKNGTC 680 KLLVATSVAA 690 RGLDVKHLIL 700 VVNYSCPNHY 710 EDYVHRAGRT 720 GRAGNKGYAY 730 TFITEDQARY 740 AGDIIKALEL 750 SGTAVPPDLE 760 KLWSDFKDQQ 770 KAEGKIIKKS 780 SGFSGKGFKF 790 DETEQALANE 800 RKKLQKAALG 810 LQDSDDEDAA 820 VDIDEQIESM 830 FNSKKRVKDM 840 AAPGTSSAPA 850 PTAGNAEKLE 860 IAKRLALRIN 870 AQKNLGIESQ 880 VDVMQQATNA 890 ILRGGPLLAP 900 TVSAKTIAEQ 910 LAEKINAKLN 920 YVPLEKQEEE 930 RQEGGQSESF 940 KRYEEELEIN 950 DFPQTARWKV 960 TSKEALQRIS 970 EYSEAAITIR 980 GTYFPPGKEP 990 KEGERKIYLA 1000 IESANELAVQ 1010 KAKAEITRLI 1020 KEELIRLQNS 1030 YQPTNKGRYK VL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005684 U2-type spliceosomal complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:1903241 U2-type prespliceosome assembly

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.