Overview
| Uniprot ID | Q62829 |
|---|
| Protein Name | Serine/threonine-protein kinase PAK 3 |
|---|
| Gene Name | Pak3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 245 |
DRQRKKSKMTDEEIL |
| 58 |
IFPGGGDKTNKKKEK |
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development (PubMed:12890786). In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity)
Protein Sequence
10
MSDSLDNEEK
20
PPAPPLRMNS
30
NNRDSSALNH
40
SSKPLPMAPE
50
EKNKKARLRS
60
IFPGGGDKTN
70
KKKEKERPEI
80
SLPSDFEHTI
90
HVGFDAVTGE
100
FTGIPEQWAR
110
LLQTSNITKL
120
EQKKNPQAVL
130
DVLKFYDSKE
140
TVNNQKYMSF
150
TSGDKSAHGY
160
IAAHQSNTKT
170
ASEPPLAPPV
180
SEEEDEEEEE
190
EEDDNEPPPV
200
IAPRPEHTKS
210
IYTRSVVESI
220
ASPAAPNKEA
230
TPPSAENANS
240
STLYRNTDRQ
250
RKKSKMTDEE
260
ILEKLRSIVS
270
VGDPKKKYTR
280
FEKIGQGASG
290
TVYTALDIAT
300
GQEVAIKQMN
310
LQQQPKKELI
320
INEILVMREN
330
KNPNIVNYLD
340
SYLVGDELWV
350
VMEYLAGGSL
360
TDVVTETCMD
370
EGQIAAVCRE
380
CLQALDFLHS
390
NQVIHRDIKS
400
DNILLGMDGS
410
VKLTDFGFCA
420
QITPEQSKRS
430
TMVGTPYWMA
440
PEVVTRKAYG
450
PKVDIWSLGI
460
MAIEMVEGEP
470
PYLNENPLRA
480
LYLIATNGTP
490
ELQNPERLSA
500
VFRDFLNRCL
510
EMDVDRRGSA
520
KELLQHPFLK
530
LAKPLSSLTP
540
LILAAKEAIK
NSSR
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005768 |
endosome |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0014069 |
postsynaptic density |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0051020 |
GTPase binding |
| Molecular Function |
GO:0004708 |
MAP kinase kinase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Molecular Function |
GO:0017124 |
SH3 domain binding |
| Molecular Function |
GO:0031267 |
small GTPase binding |
| Biological Process |
GO:0007409 |
axonogenesis |
| Biological Process |
GO:0016477 |
cell migration |
| Biological Process |
GO:0009267 |
cellular response to starvation |
| Biological Process |
GO:0016358 |
dendrite development |
| Biological Process |
GO:0060996 |
dendritic spine development |
| Biological Process |
GO:0060997 |
dendritic spine morphogenesis |
| Biological Process |
GO:0035556 |
intracellular signal transduction |
| Biological Process |
GO:0061003 |
positive regulation of dendritic spine morphogenesis |
| Biological Process |
GO:2000573 |
positive regulation of DNA biosynthetic process |
| Biological Process |
GO:0010763 |
positive regulation of fibroblast migration |
| Biological Process |
GO:0043525 |
positive regulation of neuron apoptotic process |
| Biological Process |
GO:0006468 |
protein phosphorylation |
| Biological Process |
GO:0032956 |
regulation of actin cytoskeleton organization |
| Biological Process |
GO:0030833 |
regulation of actin filament polymerization |
| Biological Process |
GO:0050770 |
regulation of axonogenesis |
| Biological Process |
GO:0043408 |
regulation of MAPK cascade |
| Biological Process |
GO:0010975 |
regulation of neuron projection development |
| Biological Process |
GO:0099175 |
regulation of postsynapse organization |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.