Overview
| Uniprot ID | Q62847 |
|---|
| Protein Name | Gamma-adducin |
|---|
| Gene Name | Add3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 470 |
RTKITWMKAEDPSKV |
| 476 |
MKAEDPSKVSSGTPI |
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin (By similarity). Involved in myogenic reactivity of the renal afferent arteriole (Af-art), renal interlobular arteries and middle cerebral artery (MCA) to increased perfusion pressure (PubMed:27927653, PubMed:32029431, PubMed:32830539). Involved in regulation of potassium channels in the vascular smooth muscle cells (VSMCs) of the Af-art and MCA ex vivo (PubMed:27927653, PubMed:32029431). Involved in regulation of glomerular capillary pressure, glomerular filtration rate (GFR) and glomerular nephrin expression in response to hypertension (PubMed:32830539, PubMed:33414130). Involved in renal blood flow (RBF) autoregulation (PubMed:32029431, PubMed:32830539, PubMed:33414130). Plays a role in podocyte structure and function. Regulates globular monomer actin (G-actin) and filamentous polymer actin (F-actin) ratios in the primary podocytes affecting actin cytoskeleton organization. Regulates expression of synaptopodin, RhoA, Rac1 and CDC42 in the renal cortex and the primary podocytes. Regulates expression of nephrin in the glomeruli and in the primary podocytes, expression of nephrin and podocinin in the renal cortex, and expression of focal adhesion proteins integrin alpha-3 and integrin beta-1 in the glomeruli. Involved in cell migration and cell adhesion of podocytes, and in podocyte foot process effacement (PubMed:33308016). Regulates expression of profibrotics markers MMP2, MMP9, TGF beta-1, tubular tight junction protein E-cadherin, and mesenchymal markers vimentin and alpha-SMA (PubMed:33414130). Promotes the growth of neurites (By similarity)
Protein Sequence
10
MSSDTSQAVI
20
TTPPPPSMPH
30
KERYFDRINE
40
SDPEYLRERN
50
MSPDLRQDFN
60
MMEQRKRVTQ
70
ILQSPAFRED
80
LECLIQEQMK
90
KGHDPTGLLA
100
LQQIADYIMA
110
NSFTGFSSPP
120
LSLGMVTPIN
130
DLPGADTSSY
140
VKGEKLTRCK
150
LASLYRLADL
160
FGWAHLANTY
170
ISVRVSKEQD
180
HIIIIPRGLS
190
FSEATASALV
200
KVNIIGEVVD
210
QGSTNLKIDH
220
SGFSPHAAIY
230
STRPDVKCVI
240
HIHTLATAAV
250
SSMKCGILPI
260
SQESLILGDV
270
AYYDYQGSLD
280
EEEERIELQK
290
VLGPSCKVLV
300
LRNHGVVALG
310
ETLEEAFHYI
320
FNVQMACEIQ
330
VQAVAGAGGV
340
DNLLILDLQK
350
YKAFTHGVAM
360
TGGGGVNMGS
370
HQKWKVGEIE
380
FEGLMRTLDN
390
LGYRTGYAYR
400
HPLVREKPRH
410
KSDVEIPATV
420
TAFSFEDDSV
430
PLSPLKYMAQ
440
RQQREKTRWL
450
NSPNTYMKVN
460
VPEESRNGET
470
SPRTKITWMK
480
AEDPSKVSSG
490
TPIKIEDPNQ
500
FVPLNTNPTE
510
VLEKRNKIRE
520
QNRYDLKTAG
530
PQSQLLAGIV
540
VDKPPSTMRF
550
EDDDQGPPAP
560
PNPFSHLMEG
570
ELEEYTKTIE
580
RKQQGLDDAE
590
QESLSDDAAS
600
VSQIQSQTQS
610
PQSVPERLEE
620
NHELFSKSFT
630
SVDVPVIVNG
640
KDEMHDVEDE
650
LAQRVSRLTT
660
STTIENIEIT
670
IKSPDRTEEV
680
LSPDGSPSKS
690
PSKKKKKFRT
700
PSFLKKNKKK
EKVEA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005903 |
brush border |
| Cellular Component |
GO:0005938 |
cell cortex |
| Cellular Component |
GO:0005911 |
cell-cell junction |
| Cellular Component |
GO:0000794 |
condensed nuclear chromosome |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005856 |
cytoskeleton |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0014069 |
postsynaptic density |
| Molecular Function |
GO:0051015 |
actin filament binding |
| Molecular Function |
GO:0005516 |
calmodulin binding |
| Molecular Function |
GO:0005080 |
protein kinase C binding |
| Molecular Function |
GO:0005200 |
structural constituent of cytoskeleton |
| Biological Process |
GO:0051016 |
barbed-end actin filament capping |
| Biological Process |
GO:0051495 |
positive regulation of cytoskeleton organization |
| Biological Process |
GO:0045907 |
positive regulation of vasoconstriction |
| Biological Process |
GO:0009410 |
response to xenobiotic stimulus |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.