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Overview

Uniprot ID	Q62848
Protein Name	ADP-ribosylation factor GTPase-activating protein 1
Gene Name	Arfgap1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
226	SKFASAAKEGATKFG
231	AAKEGATKFGSQASQ
285	KVQGVGSKGWRDVTT

Function

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine

Protein Sequence

10 MASPRTRKVL 20 KEVRAQDENN 30 VCFECGAFNP 40 QWVSVTYGIW 50 ICLECSGRHR 60 GLGVHLSFVR 70 SVTMDKWKDI 80 ELEKMKAGGN 90 AKFREFLEAQ 100 DDYEPSWSLQ 110 DKYSSRAAAL 120 FRDKVATLAE 130 GKEWSLESSP 140 AQNWTPPQPK 150 TLQFTAHRPA 160 GQPQNVTTSG 170 DKAFEDWLND 180 DLGSYQGAQE 190 NRYVGFGNTV 200 PPQKREDDFL 210 NSAMSSLYSG 220 WSSFTTGASK 230 FASAAKEGAT 240 KFGSQASQKA 250 SELGHSLNEN 260 VLKPAQEKVK 270 EGRIFDDVSS 280 GVSQLASKVQ 290 GVGSKGWRDV 300 TTFFSGKAED 310 TSDRPLEGHS 320 YQNSSGDNSQ 330 NSTIDQSFWE 340 TFGSAEPPKA 350 KSPSSDSWTC 360 ADASTGRRSS 370 DSWDIWGSGS 380 ASNNKNSNSD 390 GWESWEGASG 400 EGRAKATKKA 410 APSTAADEGW DNQNW

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0000139	Golgi membrane
Cellular Component	GO:0031965	nuclear membrane
Cellular Component	GO:0014069	postsynaptic density
Cellular Component	GO:0045202	synapse
Molecular Function	GO:0005096	GTPase activator activity
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0015031	protein transport
Biological Process	GO:0032012	regulation of ARF protein signal transduction
Biological Process	GO:0030100	regulation of endocytosis
Biological Process	GO:0016192	vesicle-mediated transport

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.