Search Results

Overview

Uniprot IDQ641Q2
Protein NameWASH complex subunit 2A
Gene NameWASHC2A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1153 ERTKPKAKIAENPAN
1166 ANPPVGGKAKSPMFP
1168 PPVGGKAKSPMFPAL
494 EGDLFKEKAVASPEA
557 SQSASKLKGASLLPG
657 TLQSQEAKAVKKTSL
906 EKKRVVKKDHSVDSF

Function

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission. Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35. Required for the endosomal recruitment of CCC complex subunits COMMD1 and CCDC93 as well as the retriever complex subunit VPS35L

Protein Sequence

10 MMNRTTPDQE 20 LAPASEPVWE 30 RPWSVEEIRR 40 SSQSWSLAAD 50 AGLLQFLQEF 60 SQQTISRTHE 70 IKKQVDGLIR 80 ETKATDCRLH 90 NVFNDFLMLS 100 NTQFIENRVY 110 DEEVEEPVLK 120 AEAEKTEQEK 130 TREQKEVDLI 140 PKVQEAVNYG 150 LQVLDSAFEQ 160 LDIKAGNSDS 170 EEDDANGRVE 180 LILEPKDLYI 190 DRPLPYLIGS 200 KLFMEQEDVG 210 LGELSSEEGS 220 VGSDRGSIVD 230 TEEEKEEEES 240 DEDFAHHSDN 250 EQNRHTTQMS 260 DEEEDDDGCD 270 LFADSEKEEE 280 DIEDIEENTR 290 PKRSRPTSFA 300 DELAARIKGD 310 AVGRVDEEPT 320 TLPSGEAKPR 330 KTLKEKKERR 340 TPSDDEEDNL 350 FAPPKLTDED 360 FSPFGSGGGL 370 FSGGKGLFDD 380 EDEESDLFTE 390 APQDRQAGAS 400 VKEESSSSKP 410 GKKIPAGAVS 420 VFLGDTDVFG 430 AASVPSMKEP 440 QKPEQPTPRK 450 SPYGPPPTGL 460 FDDDDGDDDD 470 DFFSAPHSKP 480 SKTGKVQSTA 490 DIFGDEEGDL 500 FKEKAVASPE 510 ATVSQTDENK 520 ARAEKKVTLS 530 SSKNLKPSSE 540 TKTQKGLFSD 550 EEDSEDLFSS 560 QSASKLKGAS 570 LLPGKLPTLV 580 SLFDDEDEED 590 NLFGGTAAKK 600 QTLCLQAQRE 610 EKAKASELSK 620 KKASALLFSS 630 DEEDQWNIPA 640 SQTHLASDSR 650 SKGEPRDSGT 660 LQSQEAKAVK 670 KTSLFEEDEE 680 DDLFAIAKDS 690 QKKTQRVSLL 700 FEDDVDSGGS 710 LFGSPPTSVP 720 PATKKKETVS 730 EAPPLLFSDE 740 EEKEAQLGVK 750 SVDKKVESAK 760 ESLKFGRTDV 770 AESEKEGLLT 780 RSAQETVKHS 790 DLFSSSSPWD 800 KGTKPRTKTV 810 LSLFDEEEDK 820 MEDQNIIQAP 830 QKEVGKGRDP 840 DAHPKSTGVF 850 QDEELLFSHK 860 LQKDNDPDVD 870 LFAGTKKTKL 880 LEPSVGSLFG 890 DDEDDDLFSS 900 AKSQPLVQEK 910 KRVVKKDHSV 920 DSFKNQKHPE 930 SIQGSKEKGI 940 WKPETPQDSS 950 GLAPFKTKEP 960 STRIGKIQAN 970 LAINPAALLP 980 TAASQISEVK 990 PVLPELAFPS 1000 SEHRRSHGLE 1010 SVPVLPGSGE 1020 AGVSFDLPAQ 1030 ADTLHSANKS 1040 RVKMRGKRRP 1050 QTRAARRLAA 1060 QESSETEDMS 1070 VPRGPIAQWA 1080 DGAISPNGHR 1090 PQLRAASGED 1100 STEEALAAAA 1110 APWEGGPVPG 1120 VDRSPFAKSL 1130 GHSRGEADLF 1140 DSGDIFSTGT 1150 GSQSVERTKP 1160 KAKIAENPAN 1170 PPVGGKAKSP 1180 MFPALGEASS 1190 DDDLFQSAKP 1200 KPAKKTNPFP 1210 LLEDEDDLFT 1220 DQKVKKNETK 1230 SNSQQDVILT 1240 TQDIFEDDIF 1250 ATEAIKPSQK 1260 TREKEKTLES 1270 NLFDDNIDIF 1280 ADLTVKPKEK 1290 SKKKVEAKSI 1300 FDDDMDDIFS 1310 SGIQAKTTKP 1320 KSRSAQAAPE 1330 PRFEHKVSNI 1340 FDDPLNAFGG Q

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005769 early endosome
Cellular Component GO:0031901 early endosome membrane
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0071203 WASH complex
Molecular Function GO:1901981 phosphatidylinositol phosphate binding
Molecular Function GO:1905394 retromer complex binding
Biological Process GO:0016197 endosomal transport
Biological Process GO:0036010 protein localization to endosome
Biological Process GO:0015031 protein transport
Biological Process GO:0034315 regulation of Arp2/3 complex-mediated actin nucleation
Biological Process GO:0042147 retrograde transport, endosome to Golgi

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.