Search Results

Overview

Uniprot IDQ64433
Protein Name10 kDa heat shock protein, mitochondrial
Gene NameHspe1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
28 SAAETVTKGGIMLPE
40 LPEKSQGKVLQATVV
54 VAVGSGGKGKSGEIE
56 VGSGGKGKSGEIEPV
66 EIEPVSVKVGDKVLL
70 VSVKVGDKVLLPEYG
8 MAGQAFRKFLPLFDR
80 LPEYGGTKVVLDDKD

Function

Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein

Protein Sequence

10 MAGQAFRKFL 20 PLFDRVLVER 30 SAAETVTKGG 40 IMLPEKSQGK 50 VLQATVVAVG 60 SGGKGKSGEI 70 EPVSVKVGDK 80 VLLPEYGGTK 90 VVLDDKDYFL 100 FRDSDILGKY VD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0044183 protein folding chaperone
Molecular Function GO:0051087 protein-folding chaperone binding
Molecular Function GO:0051082 unfolded protein binding
Biological Process GO:0006457 protein folding

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.