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Overview

Uniprot IDQ68E01
Protein NameIntegrator complex subunit 3
Gene NameINTS3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
106 TEPAQAQKCYRDLAL
7 *MELQKGKGAAAAAA
990 KPPKSRRKAALSSPR

Function

Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed:38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed:16239144). Within the integrator complex, INTS3 is involved in the post-termination step: INTS3 binds INTS7 in the open conformation of integrator complex and prevents the rebinding of Pol II to the integrator after termination cycle (PubMed:38570683). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed:23904267)

Protein Sequence

10 MELQKGKGAA 20 AAAAASGAAG 30 GGGGGAGAGA 40 PGGGRLLLST 50 SLDAKDELEE 60 RLERCMSIVT 70 SMTAGVSERE 80 ANDALNAYVC 90 KGLPQHEEIC 100 LGLFTLILTE 110 PAQAQKCYRD 120 LALVSRDGMN 130 IVLNKINQIL 140 MEKYLKLQDT 150 CRTQLVWLVR 160 ELVKSGVLGA 170 DGVCMTFMKQ 180 IAGGGDVTAK 190 NIWLAESVLD 200 ILTEQREWVL 210 KSSILIAMAV 220 YTYLRLIVDH 230 HGTAQLQALR 240 QKEVDFCISL 250 LRERFMECLM 260 IGRDLVRLLQ 270 NVARIPEFEL 280 LWKDIIHNPQ 290 ALSPQFTGIL 300 QLLQSRTSRK 310 FLACRLTPDM 320 ETKLLFMTSR 330 VRFGQQKRYQ 340 DWFQRQYLST 350 PDSQSLRCDL 360 IRYICGVVHP 370 SNEVLSSDIL 380 PRWAIIGWLL 390 TTCTSNVAAS 400 NAKLALFYDW 410 LFFSPDKDSI 420 MNIEPAILVM 430 HHSMKPHPAI 440 TATLLDFMCR 450 IIPNFYPPLE 460 GHVRQGVFSS 470 LNHIVEKRVL 480 AHLAPLFDNP 490 KLDKELRAML 500 REKFPEFCSS 510 PSPPVEVKIE 520 EPVSMEMDNH 530 MSDKDESCYD 540 NAEAAFSDDE 550 EDLNSKGKKR 560 EFRFHPIKET 570 VVEEPVDITP 580 YLDQLDESLR 590 DKVLQLQKGS 600 DTEAQCEVMQ 610 EIVDQVLEED 620 FDSEQLSVLA 630 SCLQELFKAH 640 FRGEVLPEEI 650 TEESLEESVG 660 KPLYLIFRNL 670 CQMQEDNSSF 680 SLLLDLLSEL 690 YQKQPKIGYH 700 LLYYLRASKA 710 AAGKMNLYES 720 FAQATQLGDL 730 HTCLMMDMKA 740 CQEDDVRLLC 750 HLTPSIYTEF 760 PDETLRSGEL 770 LNMIVAVIDS 780 AQLQELVCHV 790 MMGNLVMFRK 800 DSVLNILIQS 810 LDWETFEQYC 820 AWQLFLAHNI 830 PLETIIPILQ 840 HLKYKEHPEA 850 LSCLLLQLRR 860 EKPSEEMVKM 870 VLSRPCHPDD 880 QFTTSILRHW 890 CMKHDELLAE 900 HIKSLLIKNN 910 SLPRKRQSLR 920 SSSSKLAQLT 930 LEQILEHLDN 940 LRLNLTNTKQ 950 NFFSQTPILQ 960 ALQHVQASCD 970 EAHKMKFSDL 980 FSLAEEYEDS 990 STKPPKSRRK 1000 AALSSPRSRK 1010 NATQPPNAEE 1020 ESGSSSASEE 1030 EDTKPKPTKR 1040 KRKGSSAVGS DSD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0160232 INTAC complex
Cellular Component GO:0032039 integrator complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0035861 site of double-strand break
Cellular Component GO:0070876 SOSS complex
Biological Process GO:0006974 DNA damage response
Biological Process GO:0006281 DNA repair
Biological Process GO:0000724 double-strand break repair via homologous recombination
Biological Process GO:0044818 mitotic G2/M transition checkpoint
Biological Process GO:0034243 regulation of transcription elongation by RNA polymerase II
Biological Process GO:0010212 response to ionizing radiation
Biological Process GO:0160240 RNA polymerase II transcription initiation surveillance
Biological Process GO:0016180 snRNA processing

Reference

[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[2] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.