Overview
| Uniprot ID | Q6GQQ9 |
|---|
| Protein Name | OTU domain-containing protein 7B |
|---|
| Gene Name | OTUD7B |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 505 |
RADSVANKLGSFGKT |
| 516 |
FGKTLGSKLKKNMGG |
| 665 |
GGGPPPAKKPEPDAR |
Function
Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses (PubMed:18178551). In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B (By similarity). Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:11463333, PubMed:20622874, PubMed:23827681, PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin (PubMed:12682062). Acts as a regulator of mTORC1 and mTORC2 assembly by mediating 'Lys-63'-linked deubiquitination of MLST8, thereby promoting assembly of the mTORC2 complex, while inhibiting formation of the mTORC1 complex (PubMed:28489822)
Protein Sequence
10
MTLDMDAVLS
20
DFVRSTGAEP
30
GLARDLLEGK
40
NWDVNAALSD
50
FEQLRQVHAG
60
NLPPSFSEGS
70
GGSRTPEKGF
80
SDREPTRPPR
90
PILQRQDDIV
100
QEKRLSRGIS
110
HASSSIVSLA
120
RSHVSSNGGG
130
GGSNEHPLEM
140
PICAFQLPDL
150
TVYNEDFRSF
160
IERDLIEQSM
170
LVALEQAGRL
180
NWWVSVDPTS
190
QRLLPLATTG
200
DGNCLLHAAS
210
LGMWGFHDRD
220
LMLRKALYAL
230
MEKGVEKEAL
240
KRRWRWQQTQ
250
QNKESGLVYT
260
EDEWQKEWNE
270
LIKLASSEPR
280
MHLGTNGANC
290
GGVESSEEPV
300
YESLEEFHVF
310
VLAHVLRRPI
320
VVVADTMLRD
330
SGGEAFAPIP
340
FGGIYLPLEV
350
PASQCHRSPL
360
VLAYDQAHFS
370
ALVSMEQKEN
380
TKEQAVIPLT
390
DSEYKLLPLH
400
FAVDPGKGWE
410
WGKDDSDNVR
420
LASVILSLEV
430
KLHLLHSYMN
440
VKWIPLSSDA
450
QAPLAQPESP
460
TASAGDEPRS
470
TPESGDSDKE
480
SVGSSSTSNE
490
GGRRKEKSKR
500
DREKDKKRAD
510
SVANKLGSFG
520
KTLGSKLKKN
530
MGGLMHSKGS
540
KPGGVGTGLG
550
GSSGTETLEK
560
KKKNSLKSWK
570
GGKEEAAGDG
580
PVSEKPPAES
590
VGNGGSKYSQ
600
EVMQSLSILR
610
TAMQGEGKFI
620
FVGTLKMGHR
630
HQYQEEMIQR
640
YLSDAEERFL
650
AEQKQKEAER
660
KIMNGGIGGG
670
PPPAKKPEPD
680
AREEQPTGPP
690
AESRAMAFST
700
GYPGDFTIPR
710
PSGGGVHCQE
720
PRRQLAGGPC
730
VGGLPPYATF
740
PRQCPPGRPY
750
PHQDSIPSLE
760
PGSHSKDGLH
770
RGALLPPPYR
780
VADSYSNGYR
790
EPPEPDGWAG
800
GLRGLPPTQT
810
KCKQPNCSFY
820
GHPETNNFCS
830
CCYREELRRR
840
EREPDGELLV
HRF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Biological Process |
GO:0032717 |
negative regulation of interleukin-8 production |
| Biological Process |
GO:1900181 |
negative regulation of protein localization to nucleus |
| Biological Process |
GO:1904262 |
negative regulation of TORC1 signaling |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:1904515 |
positive regulation of TORC2 signaling |
| Biological Process |
GO:0016579 |
protein deubiquitination |
| Biological Process |
GO:0071947 |
protein deubiquitination involved in ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0035871 |
protein K11-linked deubiquitination |
| Biological Process |
GO:0071108 |
protein K48-linked deubiquitination |
| Biological Process |
GO:0070536 |
protein K63-linked deubiquitination |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0004843 |
cysteine-type deubiquitinase activity |
| Molecular Function |
GO:0008234 |
cysteine-type peptidase activity |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:1990380 |
K48-linked deubiquitinase activity |
| Molecular Function |
GO:0070530 |
K63-linked polyubiquitin modification-dependent protein binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0002250 |
adaptive immune response |
| Biological Process |
GO:0002385 |
mucosal immune response |
| Biological Process |
GO:0043124 |
negative regulation of canonical NF-kappaB signal transduction |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.