Overview
| Uniprot ID | Q6J1I8 |
|---|
| Protein Name | E3 ubiquitin-protein ligase RNF114 |
|---|
| Gene Name | RNF114 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 61 |
QECLKPKKPVCGVCR |
| 96 |
TSCHGCRKNFFLSKI |
Function
E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Also acts as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis (By similarity). Inhibits classical swine fever virus replication by mediating 'K27'-linked ubiquitination of viral NS4B and inducing its degradation via the proteasome (PubMed:31413123)
Protein Sequence
10
MAAQPQDREG
20
GAQLAGPAAE
30
ADPLGRFTCP
40
VCLEVYEKPV
50
QVPCGHVFCS
60
ACLQECLKPK
70
KPVCGVCRST
80
LAPGVRAVEL
90
ERQIESTETS
100
CHGCRKNFFL
110
SKIRAHVATC
120
SKYQNYIMEG
130
VKATTKDASL
140
QPRNVPNRYT
150
FPCPYCPEKN
160
FDQEGLVEHC
170
KLVHSTDTKS
180
VVCPICASMP
190
WGDPNYRSAN
200
FIEHIQRRHQ
210
FSYDTFVDYD
220
VDEEDMMNQV
LQRSLLDQ
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0030154 |
cell differentiation |
| Biological Process |
GO:0000209 |
protein polyubiquitination |
| Biological Process |
GO:0007283 |
spermatogenesis |
| Biological Process |
GO:0006511 |
ubiquitin-dependent protein catabolic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.