Search Results

Overview

Uniprot ID	Q6P2C8
Protein Name	Mediator of RNA polymerase II transcription subunit 27
Gene Name	MED27
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
145	NQMGVSAKRRPKAQP
149	VSAKRRPKAQPTTLV

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

Protein Sequence

10 MADVINVSVN 20 LEAFSQAISA 30 IQALRSSVSR 40 VFDCLKDGMR 50 NKETLEGREK 60 AFIAHFQDNL 70 HSVNRDLNEL 80 ERLSNLVGKP 90 SENHPLHNSG 100 LLSLDPVQDK 110 TPLYSQLLQA 120 YKWSNKLQYH 130 AGLASGLLNQ 140 QSLKRSANQM 150 GVSAKRRPKA 160 QPTTLVLPPQ 170 YVDDVISRID 180 RMFPEMSIHL 190 SRPNGTSAML 200 LVTLGKVLKV 210 IVVMRSLFID 220 RTIVKGYNEN 230 VYTEDGKLDI 240 WSKSNYQVFQ 250 KVTDHATTAL 260 LHYQLPQMPD 270 VVVRSFMTWL 280 RSYIKLFQAP 290 CQRCGKFLQD 300 GLPPTWRDFR 310 TLEAFHDTCR Q

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0016592	mediator complex
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005667	transcription regulator complex
Cellular Component	GO:0000151	ubiquitin ligase complex
Molecular Function	GO:0003713	transcription coactivator activity
Molecular Function	GO:0061630	ubiquitin protein ligase activity
Biological Process	GO:0032968	positive regulation of transcription elongation by RNA polymerase II
Biological Process	GO:0060261	positive regulation of transcription initiation by RNA polymerase II
Biological Process	GO:0016567	protein ubiquitination
Biological Process	GO:0006357	regulation of transcription by RNA polymerase II
Biological Process	GO:0051123	RNA polymerase II preinitiation complex assembly
Biological Process	GO:0006367	transcription initiation at RNA polymerase II promoter

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.