Search Results

Overview

Uniprot ID	Q6PD62
Protein Name	RNA polymerase-associated protein CTR9 homolog
Gene Name	CTR9
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1005	ERLPPSMKGKIKSKA
1007	LPPSMKGKIKSKAII
637	DRALAIYKQVLRNDA
884	QRAQYVEKTKNILMF
913	GGGRRSKKGGEFDEF

Function

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3 (By similarity)

Protein Sequence

10 MSRGSIEIPL 20 RDTDEVIELD 30 FDQLPEGDEV 40 ISILKQEHTQ 50 LHIWIALALE 60 YYKQGKTEEF 70 VKLLEAARID 80 GNLDYRDHEK 90 DQMTCLDTLA 100 AYYVQQARKE 110 KNKDNKKDLI 120 TQATLLYTMA 130 DKIIMYDQNH 140 LLGRACFCLL 150 EGDKMDQADA 160 QFHFVLNQSP 170 NNIPALLGKA 180 CISFNKKDYR 190 GALAYYKKAL 200 RTNPGCPAEV 210 RLGMGHCFVK 220 LNKLEKARLA 230 FSRALELNSK 240 CVGALVGLAV 250 LELNNKEADS 260 IKNGVQLLSR 270 AYTIDPSNPM 280 VLNHLANHFF 290 FKKDYSKVQH 300 LALHAFHNTE 310 VEAMQAESCY 320 QLARSFHVQE 330 DYDQAFQYYY 340 QATQFASSSF 350 VLPFFGLGQM 360 YIYRGDKENA 370 SQCFEKVLKA 380 YPNNYETMKI 390 LGSLYAASED 400 QEKRDIAKGH 410 LKKVTEQYPD 420 DVEAWIELAQ 430 ILEQTDIQGA 440 LSAYGTATRI 450 LQEKVQADVP 460 PEILNNVGAL 470 HFRLGNLGEA 480 KKYFLASLDR 490 AKAEAEHDEH 500 YYNAISVTTS 510 YNLARLYEAM 520 CEFHEAEKLY 530 KNILREHPNY 540 VDCYLRLGAM 550 ARDKGNFYEA 560 SDWFKEALQI 570 NQDHPDAWSL 580 IGNLHLAKQE 590 WGPGQKKFER 600 ILKQPSTQSD 610 TYSMLALGNV 620 WLQTLHQPTR 630 DREKEKRHQD 640 RALAIYKQVL 650 RNDAKNLYAA 660 NGIGAVLAHK 670 GYFREARDVF 680 AQVREATADI 690 SDVWLNLAHI 700 YVEQKQYISA 710 VQMYENCLRK 720 FYKHQNTEVV 730 LYLARALFKC 740 GKLQECKQTL 750 LKARHVAPSD 760 TVLMFNVALV 770 LQRLATSVLK 780 DEKSNLKEVL 790 NAVKELELAH 800 RYFSYLSKVG 810 DKMRFDLALA 820 ATEARQCSDL 830 LSQAQYHVAR 840 ARKQDEEERE 850 LRAKQEQEKE 860 LLRQKLLKEQ 870 EEKRLREKEE 880 QKKLLEQRAQ 890 YVEKTKNILM 900 FTGETEATKE 910 KKRGGGGGRR 920 SKKGGEFDEF 930 VNDDTDDDLP 940 ISKKKKRRKG 950 SGSEQEGEDE 960 EGGERKKKKR 970 RRHPKGEEGS 980 DDDETENGPK 990 PKKRRPPKAE 1000 KKKAPKPERL 1010 PPSMKGKIKS 1020 KAIISSSDDS 1030 SDEDKLKIAD 1040 EGHPRNSNSN 1050 SDSDEDEQRK 1060 KCASSESDSD 1070 ENQNKSGSEA 1080 GSPRRPRRQR 1090 SDQDSDSDQP 1100 SRKRRPSGSE 1110 QSDNESVQSG 1120 RSHSGVSEND 1130 SRPASPSAES 1140 DHESERGSDN 1150 EGSGQGSGNE 1160 SEPEGSNNEA 1170 SDRGSEHGSD DSD

Gene Ontology

Classification	GO ID	Description
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:0045944	positive regulation of transcription by RNA polymerase II
Biological Process	GO:0019827	stem cell population maintenance
Biological Process	GO:0006368	transcription elongation by RNA polymerase II
Biological Process	GO:0016055	Wnt signaling pathway
Cellular Component	GO:0016593	Cdc73/Paf1 complex
Cellular Component	GO:0000791	euchromatin
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0000993	RNA polymerase II complex binding
Molecular Function	GO:0042169	SH2 domain binding
Biological Process	GO:0007259	cell surface receptor signaling pathway via JAK-STAT
Biological Process	GO:0071222	cellular response to lipopolysaccharide
Biological Process	GO:0006325	chromatin organization
Biological Process	GO:0001711	endodermal cell fate commitment
Biological Process	GO:0070102	interleukin-6-mediated signaling pathway
Biological Process	GO:0045638	negative regulation of myeloid cell differentiation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.