Overview
| Uniprot ID | Q6PJ21 |
|---|
| Protein Name | SPRY domain-containing SOCS box protein 3 |
|---|
| Gene Name | SPSB3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 238 |
LTFFKNRKCIGVAAT |
Function
Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as CGAS and SNAI1 (PubMed:29059170, PubMed:38418882). The ECS(SPSB3) complex catalyzes 'Lys-48'-linked ubiquitination of nuclear CGAS in cycling cells, leading to its degradation (PubMed:38418882). Recognizes and binds nucleosome-bound CGAS: ubiquitination and degradation of nuclear CGAS during G1 and G2 phases is required to promote low intranuclear CGAS abundance before the next mitotic cycle (PubMed:38418882). The ECS(SPSB3) complex also mediates ubiquitination and degradation of phosphorylated SNAI1 (PubMed:29059170)
Protein Sequence
10
MARRPRNSRA
20
WHFVLSAARR
30
DADARAVALA
40
GSTNWGYDSD
50
GQHSDSDSDP
60
EYSTLPPSIP
70
SAVPVTGESF
80
CDCAGQSEAS
90
FCSSLHSAHR
100
GRDCRCGEED
110
EYFDWVWDDL
120
NKSSATLLSC
130
DNRKVSFHME
140
YSCGTAAIRG
150
TKELGEGQHF
160
WEIKMTSPVY
170
GTDMMVGIGT
180
SDVDLDKYRH
190
TFCSLLGRDE
200
DSWGLSYTGL
210
LHHKGDKTSF
220
SSRFGQGSII
230
GVHLDTWHGT
240
LTFFKNRKCI
250
GVAATKLQNK
260
RFYPMVCSTA
270
ARSSMKVTRS
280
CASATSLQYL
290
CCHRLRQLRP
300
DSGDTLEGLP
310
LPPGLKQVLH
320
NKLGWVLSMS
330
CSRRKAPVSD
340
PQAATSAHPS
350
SREPRPCQRK
RCRRT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0031466 |
Cul5-RING ubiquitin ligase complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0019005 |
SCF ubiquitin ligase complex |
| Molecular Function |
GO:1990756 |
ubiquitin-like ligase-substrate adaptor activity |
| Biological Process |
GO:0160049 |
negative regulation of cGAS/STING signaling pathway |
| Biological Process |
GO:0010719 |
negative regulation of epithelial to mesenchymal transition |
| Biological Process |
GO:0043161 |
proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0070936 |
protein K48-linked ubiquitination |
Reference
PMID: N/A