Search Results

Overview

Uniprot ID	Q6PKG0
Protein Name	La-related protein 1
Gene Name	LARP1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
160	VVRAAVPKQRKGSKV
251	KKKGNKHKWVPLQID
367	DYQFGYRKFDGVEGP
543	SNLKTLPKGLSASLP

Function

RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:26206669, PubMed:28379136, PubMed:28650797, PubMed:29244122). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28379136, PubMed:28650797, PubMed:29244122). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543)

Protein Sequence

10 MATQVEPLLP 20 GGATLLQAEE 30 HGGLVRKKPP 40 PAPEGKGEPG 50 PNDVRGGEPD 60 GSARRPRPPC 70 AKPHKEGTGQ 80 QERESPRPLQ 90 LPGAEGPAIS 100 DGEEGGGEPG 110 AGGGAAGAAG 120 AGRRDFVEAP 130 PPKVNPWTKN 140 ALPPVLTTVN 150 GQSPPEHSAP 160 AKVVRAAVPK 170 QRKGSKVGDF 180 GDAINWPTPG 190 EIAHKSVQPQ 200 SHKPQPTRKL 210 PPKKDMKEQE 220 KGEGSDSKES 230 PKTKSDESGE 240 EKNGDEDCQR 250 GGQKKKGNKH 260 KWVPLQIDMK 270 PEVPREKLAS 280 RPTRPPEPRH 290 IPANRGEIKG 300 SESATYVPVA 310 PPTPAWQPEI 320 KPEPAWHDQD 330 ETSSVKSDGA 340 GGARASFRGR 350 GRGRGRGRGR 360 GRGGTRTHFD 370 YQFGYRKFDG 380 VEGPRTPKYM 390 NNITYYFDNV 400 SSTELYSVDQ 410 ELLKDYIKRQ 420 IEYYFSVDNL 430 ERDFFLRRKM 440 DADGFLPITL 450 IASFHRVQAL 460 TTDISLIFAA 470 LKDSKVVEIV 480 DEKVRRREEP 490 EKWPLPPIVD 500 YSQTDFSQLL 510 NCPEFVPRQH 520 YQKETESAPG 530 SPRAVTPVPT 540 KTEEVSNLKT 550 LPKGLSASLP 560 DLDSENWIEV 570 KKRPRPSPAR 580 PKKSEESRFS 590 HLTSLPQQLP 600 SQQLMSKDQD 610 EQEELDFLFD 620 EEMEQMDGRK 630 NTFTAWSDEE 640 SDYEIDDRDV 650 NKILIVTQTP 660 HYMRRHPGGD 670 RTGNHTSRAK 680 MSAELAKVIN 690 DGLFYYEQDL 700 WAEKFEPEYS 710 QIKQEVENFK 720 KVNMISREQF 730 DTLTPEPPVD 740 PNQEVPPGPP 750 RFQQVPTDAL 760 ANKLFGAPEP 770 STIARSLPTT 780 VPESPNYRNT 790 RTPRTPRTPQ 800 LKDSSQTSRF 810 YPVVKEGRTL 820 DAKMPRKRKT 830 RHSSNPPLES 840 HVGWVMDSRE 850 HRPRTASISS 860 SPSEGTPTVG 870 SYGCTPQSLP 880 KFQHPSHELL 890 KENGFTQHVY 900 HKYRRRCLNE 910 RKRLGIGQSQ 920 EMNTLFRFWS 930 FFLRDHFNKK 940 MYEEFKQLAL 950 EDAKEGYRYG 960 LECLFRYYSY 970 GLEKKFRLDI 980 FKDFQEETVK 990 DYEAGQLYGL 1000 EKFWAFLKYS 1010 KAKNLDIDPK 1020 LQEYLGKFRR 1030 LEDFRVDPPM 1040 GEEGNHKRHS 1050 VVAGGGGGEG 1060 RKRCPSQSSS 1070 RPAAMISQPP 1080 TPPTGQPVRE 1090 DAKWTSQHSN TQTLGK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0010494	cytoplasmic stress granule
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016020	membrane
Molecular Function	GO:0045296	cadherin binding
Molecular Function	GO:0008190	eukaryotic initiation factor 4E binding
Molecular Function	GO:0003730	mRNA 3'-UTR binding
Molecular Function	GO:0048027	mRNA 5'-UTR binding
Molecular Function	GO:0043024	ribosomal small subunit binding
Molecular Function	GO:0000340	RNA 7-methylguanosine cap binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0000339	RNA cap binding
Molecular Function	GO:0008494	translation activator activity
Molecular Function	GO:0031369	translation initiation factor binding
Biological Process	GO:0008283	cell population proliferation
Biological Process	GO:0072752	cellular response to rapamycin
Biological Process	GO:0048255	mRNA stabilization
Biological Process	GO:0017148	negative regulation of translation
Biological Process	GO:0045947	negative regulation of translational initiation
Biological Process	GO:0016239	positive regulation of macroautophagy
Biological Process	GO:0045727	positive regulation of translation
Biological Process	GO:0045948	positive regulation of translational initiation
Biological Process	GO:0045070	positive regulation of viral genome replication
Biological Process	GO:0010608	post-transcriptional regulation of gene expression
Biological Process	GO:1990928	response to amino acid starvation
Biological Process	GO:0031929	TOR signaling
Biological Process	GO:0038202	TORC1 signaling
Biological Process	GO:0006413	translational initiation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.