Search Results

Overview

Uniprot ID	Q6R327
Protein Name	Rapamycin-insensitive companion of mTOR
Gene Name	RICTOR
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
124	LQKVLKLKVDYLIAR
719	LARVILSKILTAATD

Function

Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed:15268862, PubMed:15718470, PubMed:19720745, PubMed:19995915, PubMed:21343617, PubMed:33158864, PubMed:35904232, PubMed:35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed:19720745, PubMed:19935711, PubMed:19995915). In contrast to mTORC1, mTORC2 is nutrient-insensitive (PubMed:15467718, PubMed:21343617). Within the mTORC2 complex, RICTOR probably acts as a molecular adapter (PubMed:21343617, PubMed:33158864, PubMed:35926713). RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2 (PubMed:33158864). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed:15718470, PubMed:19720745, PubMed:19935711, PubMed:35926713). mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (By similarity). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed:15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed:15467718)

Protein Sequence

10 MAAIGRGRSL 20 KNLRVRGRND 30 SGEENVPLDL 40 TREPSDNLRE 50 ILQNVARLQG 60 VSNMRKLGHL 70 NNFTKLLCDI 80 GHSEEKLGFH 90 YEDIIICLRL 100 ALLNEAKEVR 110 AAGLRALRYL 120 IQDSSILQKV 130 LKLKVDYLIA 140 RCIDIQQSNE 150 VERTQALRLV 160 RKMITVNASL 170 FPSSVTNSLI 180 AVGNDGLQER 190 DRMVRACIAI 200 ICELALQNPE 210 VVALRGGLNT 220 ILKNVIDCQL 230 SRINEALITT 240 ILHLLNHPKT 250 RQYVRADVEL 260 ERILAPYTDF 270 HYRHSPDTAE 280 GQLKEDREAR 290 FLASKMGIIA 300 TFRSWAGIIN 310 LCKPGNSGIQ 320 SLIGVLCIPN 330 MEIRRGLLEV 340 LYDIFRLPLP 350 VVTEEFIEAL 360 LSVDPGRFQD 370 SWRLSDGFVA 380 AEAKTILPHR 390 ARSRPDLMDN 400 YLALILSAFI 410 RNGLLEGLVE 420 VITNSDDHIS 430 VRATILLGEL 440 LHMANTILPH 450 SHSHHLHCLP 460 TLMNMAASFD 470 IPKEKRLRAS 480 AALNCLKRFH 490 EMKKRGPKPY 500 SLHLDHIIQK 510 AIATHQKRDQ 520 YLRVQKDIFI 530 LKDTEEALLI 540 NLRDSQVLQH 550 KENLEWNWNL 560 IGTILKWPNV 570 NLRNYKDEQL 580 HRFVRRLLYF 590 YKPSSKLYAN 600 LDLDFAKAKQ 610 LTVVGCQFTE 620 FLLESEEDGQ 630 GYLEDLVKDI 640 VQWLNASSGM 650 KPERSLQNNG 660 LLTTLSQHYF 670 LFIGTLSCHP 680 HGVKMLEKCS 690 VFQCLLNLCS 700 LKNQDHLLKL 710 TVSSLDYSRD 720 GLARVILSKI 730 LTAATDACRL 740 YATKHLRVLL 750 RANVEFFNNW 760 GIELLVTQLH 770 DKNKTISSEA 780 LDILDEACED 790 KANLHALIQM 800 KPALSHLGDK 810 GLLLLLRFLS 820 IPKGFSYLNE 830 RGYVAKQLEK 840 WHREYNSKYV 850 DLIEEQLNEA 860 LTTYRKPVDG 870 DNYVRRSNQR 880 LQRPHVYLPI 890 HLYGQLVHHK 900 TGCHLLEVQN 910 IITELCRNVR 920 TPDLDKWEEI 930 KKLKASLWAL 940 GNIGSSNWGL 950 NLLQEENVIP 960 DILKLAKQCE 970 VLSIRGTCVY 980 VLGLIAKTKQ 990 GCDILKCHNW 1000 DAVRHSRKHL 1010 WPVVPDDVEQ 1020 LCNELSSIPS 1030 TLSLNSESTS 1040 SRHNSESESV 1050 PSSMFILEDD 1060 RFGSSSTSTF 1070 FLDINEDTEP 1080 TFYDRSGPIK 1090 DKNSFPFFAS 1100 SKLVKNRILN 1110 SLTLPNKKHR 1120 SSSDPKGGKL 1130 SSESKTSNRR 1140 IRTLTEPSVD 1150 FNHSDDFTPI 1160 STVQKTLQLE 1170 TSFMGNKHIE 1180 DTGSTPSIGE 1190 NDLKFTKNFG 1200 TENHRENTSR 1210 ERLVVESSTS 1220 SHMKIRSQSF 1230 NTDTTTSGIS 1240 SMSSSPSRET 1250 VGVDATTMDT 1260 DCGSMSTVVS 1270 TKTIKTSHYL 1280 TPQSNHLSLS 1290 KSNSVSLVPP 1300 GSSHTLPRRA 1310 QSLKAPSIAT 1320 IKSLADCNFS 1330 YTSSRDAFGY 1340 ATLKRLQQQR 1350 MHPSLSHSEA 1360 LASPAKDVLF 1370 TDTITMKANS 1380 FESRLTPSRF 1390 MKALSYASLD 1400 KEDLLSPINQ 1410 NTLQRSSSVR 1420 SMVSSATYGG 1430 SDDYIGLALP 1440 VDINDIFQVK 1450 DIPYFQTKNI 1460 PPHDDRGARA 1470 FAHDAGGLPS 1480 GTGGLVKNSF 1490 HLLRQQMSLT 1500 EIMNSIHSDA 1510 SLFLESTEDT 1520 GLQEHTDDNC 1530 LYCVCIEILG 1540 FQPSNQLSAI 1550 CSHSDFQDIP 1560 YSDWCEQTIH 1570 NPLEVVPSKF 1580 SGISGCSDGV 1590 SQEGSASSTK 1600 STELLLGVKT 1610 IPDDTPMCRI 1620 LLRKEVLRLV 1630 INLSSSVSTK 1640 CHETGLLTIK 1650 EKYPQTFDDI 1660 CLYSEVSHLL 1670 SHCTFRLPCR 1680 RFIQELFQDV 1690 QFLQMHEEAE 1700 AVLATPPKQP IVDTSAES

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0005765	lysosomal membrane
Cellular Component	GO:0005764	lysosome
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0031932	TORC2 complex
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0140767	enzyme-substrate adaptor activity
Molecular Function	GO:0060090	molecular adaptor activity
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0043539	protein serine/threonine kinase activator activity
Molecular Function	GO:0043022	ribosome binding
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0030036	actin cytoskeleton organization
Biological Process	GO:0031669	cellular response to nutrient levels
Biological Process	GO:0007010	cytoskeleton organization
Biological Process	GO:0009792	embryo development ending in birth or egg hatching
Biological Process	GO:0008610	lipid biosynthetic process
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0030307	positive regulation of cell growth
Biological Process	GO:0030335	positive regulation of cell migration
Biological Process	GO:0001938	positive regulation of endothelial cell proliferation
Biological Process	GO:0051897	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Process	GO:0032008	positive regulation of TOR signaling
Biological Process	GO:0032956	regulation of actin cytoskeleton organization
Biological Process	GO:0051896	regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Process	GO:0038203	TORC2 signaling

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.