Search Results

Overview

Uniprot IDQ6Y7W6
Protein NameGRB10-interacting GYF protein 2
Gene NameGIGYF2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1103 KNNASLSKSVGVSNR
175 RFEKPGRKDVGRPNF
985 QQQQQQQKLSGWGNV

Function

Key component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:22751931, PubMed:31439631, PubMed:35878012). In the 4EHP-GYF2 complex, acts as a factor that bridges EIF4E2 to ZFP36/TTP, linking translation repression with mRNA decay (PubMed:31439631). Also recruits and bridges the association of the 4EHP complex with the decapping effector protein DDX6, which is required for the ZFP36/TTP-mediated down-regulation of AU-rich mRNA (PubMed:31439631). May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling, including IGF1 and insulin receptors (PubMed:12771153). In association with EIF4E2, assists ribosome-associated quality control (RQC) by sequestering the mRNA cap, blocking ribosome initiation and decreasing the translational load on problematic messages. Part of a pathway that works in parallel to RQC-mediated degradation of the stalled nascent polypeptide (PubMed:32726578). GIGYF2 and EIF4E2 work downstream and independently of ZNF598, which seems to work as a scaffold that can recruit them to faulty mRNA even if alternative recruitment mechanisms may exist (PubMed:32726578)

Protein Sequence

10 MAAETQTLNF 20 GPEWLRALSS 30 GGSITSPPLS 40 PALPKYKLAD 50 YRYGREEMLA 60 LFLKDNKIPS 70 DLLDKEFLPI 80 LQEEPLPPLA 90 LVPFTEEEQR 100 NFSMSVNSAA 110 VLRLTGRGGG 120 GTVVGAPRGR 130 SSSRGRGRGR 140 GECGFYQRSF 150 DEVEGVFGRG 160 GGREMHRSQS 170 WEERGDRRFE 180 KPGRKDVGRP 190 NFEEGGPTSV 200 GRKHEFIRSE 210 SENWRIFREE 220 QNGEDEDGGW 230 RLAGSRRDGE 240 RWRPHSPDGP 250 RSAGWREHME 260 RRRRFEFDFR 270 DRDDERGYRR 280 VRSGSGSIDD 290 DRDSLPEWCL 300 EDAEEEMGTF 310 DSSGAFLSLK 320 KVQKEPIPEE 330 QEMDFRPVDE 340 GEECSDSEGS 350 HNEEAKEPDK 360 TNKKEGEKTD 370 RVGVEASEET 380 PQTSSSSARP 390 GTPSDHQSQE 400 ASQFERKDEP 410 KTEQTEKAEE 420 ETRMENSLPA 430 KVPSRGDEMV 440 ADVQQPLSQI 450 PSDTASPLLI 460 LPPPVPNPSP 470 TLRPVETPVV 480 GAPGMGSVST 490 EPDDEEGLKH 500 LEQQAEKMVA 510 YLQDSALDDE 520 RLASKLQEHR 530 AKGVSIPLMH 540 EAMQKWYYKD 550 PQGEIQGPFN 560 NQEMAEWFQA 570 GYFTMSLLVK 580 RACDESFQPL 590 GDIMKMWGRV 600 PFSPGPAPPP 610 HMGELDQERL 620 TRQQELTALY 630 QMQHLQYQQF 640 LIQQQYAQVL 650 AQQQKAALSS 660 QQQQQLALLL 670 QQFQTLKMRI 680 SDQNIIPSVT 690 RSVSVPDTGS 700 IWELQPTASQ 710 PTVWEGGSVW 720 DLPLDTTTPG 730 PALEQLQQLE 740 KAKAAKLEQE 750 RREAEMRAKR 760 EEEERKRQEE 770 LRRQQEEILR 780 RQQEEERKRR 790 EEEELARRKQ 800 EEALRRQREQ 810 EIALRRQREE 820 EERQQQEEAL 830 RRLEERRREE 840 EERRKQEELL 850 RKQEEEAAKW 860 AREEEEAQRR 870 LEENRLRMEE 880 EAARLRHEEE 890 ERKRKELEVQ 900 RQKELMRQRQ 910 QQQEALRRLQ 920 QQQQQQQLAQ 930 MKLPSSSTWG 940 QQSNTTACQS 950 QATLSLAEIQ 960 KLEEERERQL 970 REEQRRQQRE 980 LMKALQQQQQ 990 QQQQKLSGWG 1000 NVSKPSGTTK 1010 SLLEIQQEEA 1020 RQMQKQQQQQ 1030 QQHQQPNRAR 1040 NNTHSNLHTS 1050 IGNSVWGSIN 1060 TGPPNQWASD 1070 LVSSIWSNAD 1080 TKNSNMGFWD 1090 DAVKEVGPRN 1100 STNKNKNNAS 1110 LSKSVGVSNR 1120 QNKKVEEEEK 1130 LLKLFQGVNK 1140 AQDGFTQWCE 1150 QMLHALNTAN 1160 NLDVPTFVSF 1170 LKEVESPYEV 1180 HDYIRAYLGD 1190 TSEAKEFAKQ 1200 FLERRAKQKA 1210 NQQRQQQQLP 1220 QQQQQQPPQQ 1230 PPQQPQQQDS 1240 VWGMNHSTLH 1250 SVFQTNQSNN 1260 QQSNFEAVQS 1270 GKKKKKQKMV 1280 RADPSLLGFS 1290 VNASSERLNM GEIETLDDY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0010494 cytoplasmic stress granule
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005768 endosome
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0016020 membrane
Cellular Component GO:0043204 perikaryon
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:1990635 proximal dendrite
Cellular Component GO:0031982 vesicle
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0000900 mRNA regulatory element binding translation repressor activity
Molecular Function GO:0070064 proline-rich region binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0048009 insulin-like growth factor receptor signaling pathway
Biological Process GO:0061157 mRNA destabilization
Biological Process GO:0017148 negative regulation of translation
Biological Process GO:0045947 negative regulation of translational initiation
Biological Process GO:0060339 negative regulation of type I interferon-mediated signaling pathway
Biological Process GO:0016441 post-transcriptional gene silencing
Biological Process GO:1990261 pre-mRNA catabolic process
Biological Process GO:0072344 rescue of stalled cytosolic ribosome

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.