Search Results

Overview

Uniprot IDQ6ZNA4
Protein NameE3 ubiquitin-protein ligase Arkadia
Gene NameRNF111
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
913 TYPHKYKKVTTDWFS

Function

E3 ubiquitin-protein ligase (PubMed:26656854). Required for mesoderm patterning during embryonic development (By similarity). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019, PubMed:16601693). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019, PubMed:16601693). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (PubMed:17591695). Associates with UBE2D2 as an E2 enzyme (PubMed:22411132). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23751493). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (PubMed:23751493). Mediates ubiquitination and degradation of sumoylated PML (By similarity). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity)

Protein Sequence

10 MSQWTPEYNE 20 LYTLKVDMKS 30 EIPSDAPKTQ 40 ESLKGILLHP 50 EPIGAAKSFP 60 AGVEMINSKV 70 GNEFSHLCDD 80 SQKQEKEMNG 90 NQQEQEKSLV 100 VRKKRKSQQA 110 GPSYVQNCVK 120 ENQGILGLRQ 130 HLGTPSDEDN 140 DSSFSDCLSS 150 PSSSLHFGDS 160 DTVTSDEDKE 170 VSVRHSQTIL 180 NAKSRSHSAR 190 SHKWPRTETE 200 SVSGLLMKRP 210 CLHGSSLRRL 220 PCRKRFVKNN 230 SSQRTQKQKE 240 RILMQRKKRE 250 VLARRKYALL 260 PSSSSSSEND 270 LSSESSSSSS 280 TEGEEDLFVS 290 ASENHQNNPA 300 VPSGSIDEDV 310 VVIEASSTPQ 320 VTANEEINVT 330 STDSEVEIVT 340 VGESYRSRST 350 LGHSRSHWSQ 360 GSSSHASRPQ 370 EPRNRSRIST 380 VIQPLRQNAA 390 EVVDLTVDED 400 EPTVVPTTSA 410 RMESQATSAS 420 INNSNPSTSE 430 QASDTASAVT 440 SSQPSTVSET 450 SATLTSNSTT 460 GTSIGDDSRR 470 TTSSAVTETG 480 PPAMPRLPSC 490 CPQHSPCGGS 500 SQNHHALGHP 510 HTSCFQQHGH 520 HFQHHHHHHH 530 TPHPAVPVSP 540 SFSDPACPVE 550 RPPQVQAPCG 560 ANSSSGTSYH 570 EQQALPVDLS 580 NSGIRSHGSG 590 SFHGASAFDP 600 CCPVSSSRAA 610 IFGHQAAAAA 620 PSQPLSSIDG 630 YGSSMVAQPQ 640 PQPPPQPSLS 650 SCRHYMPPPY 660 ASLTRPLHHQ 670 ASACPHSHGN 680 PPPQTQPPPQ 690 VDYVIPHPVH 700 AFHSQISSHA 710 TSHPVAPPPP 720 THLASTAAPI 730 PQHLPPTHQP 740 ISHHIPATAP 750 PAQRLHPHEV 760 MQRMEVQRRR 770 MMQHPTRAHE 780 RPPPHPHRMH 790 PNYGHGHHIH 800 VPQTMSSHPR 810 QAPERSAWEL 820 GIEAGVTAAT 830 YTPGALHPHL 840 AHYHAPPRLH 850 HLQLGALPLM 860 VPDMAGYPHI 870 RYISSGLDGT 880 SFRGPFRGNF 890 EELIHLEERL 900 GNVNRGASQG 910 TIERCTYPHK 920 YKKVTTDWFS 930 QRKLHCKQDG 940 EEGTEEDTEE 950 KCTICLSILE 960 EGEDVRRLPC 970 MHLFHQVCVD 980 QWLITNKKCP 990 ICRVDIEAQL PSES

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0016605 PML body
Cellular Component GO:0032991 protein-containing complex
Molecular Function GO:0046332 SMAD binding
Molecular Function GO:0032184 SUMO polymer binding
Molecular Function GO:0061630 ubiquitin protein ligase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0070911 global genome nucleotide-excision repair
Biological Process GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

PMID: N/A