Overview
| Uniprot ID | Q6ZNE9 |
|---|
| Protein Name | RUN and FYVE domain-containing protein 4 |
|---|
| Gene Name | RUFY4 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 9 |
AEEGAILKVTKDLRA |
Function
ARL8 effector that promotes the coupling of endolysosomes to dynein-dynactin for retrograde transport along microtubules. Acts by binding both GTP-bound ARL8 and dynein-dynactin. In nonneuronal cells, promotes concentration of endolysosomes in the juxtanuclear area. In hippocampal neurons, drives retrograde transport of endolysosomes from the axon to the soma (PubMed:35314674). Positive regulator of macroautophagy in dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger (PubMed:26416964). Positive regulator of osteoclast bone-resorbing activity, possibly by promoting late endosome-lysosome fusion by acting as an adapter protein between RAB7A on late endosomes and LAMP2 on primary lysosomes (By similarity)
Protein Sequence
10
MAEEGAILKV
20
TKDLRAAVSA
30
ILQGYGDGQG
40
PVTDTSAELH
50
RLCGCLELLL
60
QFDQKEQKSF
70
LGPRKDYWDF
80
LCTALRRQRG
90
NMEPIHFVRS
100
QDKLKTPLGK
110
GRAFIRFCLA
120
RGQLAEALQL
130
CLLNSELTRE
140
WYGPRSPLLC
150
PERQEDILDS
160
LYALNGVAFE
170
LDLQQPDLDG
180
AWPMFSESRC
190
SSSTQTQGRR
200
PRKNKDAPKK
210
IPAAYGGPEN
220
VQIEDSHTSQ
230
AICLQDAPSG
240
QQLAGLPRSQ
250
QQRHLPFFLE
260
KKGESSRKHR
270
YPQSMWEPEG
280
KELQLDQEER
290
APWIEIFLGN
300
STPSTQGQGK
310
GAMGTQKEVI
320
GMEAEVTGVL
330
LVAEGQRTTE
340
GTHKKEAEWS
350
HVQRLLMPSP
360
RGAVEGAVSG
370
SRQGSGGSSI
380
LGEPWVLQGH
390
ATKEDSTVEN
400
PQVQTEVTLV
410
ARREEQAEVS
420
LQDEIKSLRL
430
GLRKAEEQAQ
440
RQEQLLREQE
450
GELQALREQL
460
SRCQEERAEL
470
QAQLEQKQQE
480
AERRDAMYQE
490
ELGGQRDLVQ
500
AMKRRVLELI
510
QEKDRLWQRL
520
QHLSSMAPEC
530
CVACSKIFGR
540
FSRRYPCRLC
550
GGLLCHACSM
560
DYKKRDRCCP
570
PCAQGREAQV
T
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005776 |
autophagosome |
| Molecular Function |
GO:0032266 |
phosphatidylinositol-3-phosphate binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0000045 |
autophagosome assembly |
| Biological Process |
GO:0071353 |
cellular response to interleukin-4 |
| Biological Process |
GO:0061763 |
multivesicular body-lysosome fusion |
| Biological Process |
GO:0045780 |
positive regulation of bone resorption |
| Biological Process |
GO:0045921 |
positive regulation of exocytosis |
| Biological Process |
GO:0016239 |
positive regulation of macroautophagy |
| Biological Process |
GO:2001019 |
positive regulation of retrograde axon cargo transport |
| Cellular Component |
GO:0036019 |
endolysosome |
| Molecular Function |
GO:0034452 |
dynactin binding |
Reference
[1] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.