Overview
| Uniprot ID | Q6ZW49 |
|---|
| Protein Name | PAX-interacting protein 1 |
|---|
| Gene Name | PAXIP1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 278 |
VPQLAAAKRRLPQGK |
| 847 |
ANVQPSSKRARIEDV |
Function
Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Proposed to recruit PAGR1 to sites of DNA damage and the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probably independent of MLL-containing histone methyltransferase (HMT) complexes. However, this function has been questioned (By similarity). Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by KMT2D/MLL2. During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes. Conflictingly, its function in transcriptional regulation during immunoglobulin class switching is reported to be independent of the MLL2/MLL3 complex (By similarity)
Protein Sequence
10
MSDQAPKVPE
20
EMFREVKYYA
30
VGDIDPQVIQ
40
LLKAGKAKEV
50
SYNALASHII
60
SEDGDNPEVG
70
EAREVFDLPV
80
VKPSWVILSV
90
QCGTLLPVNG
100
FSPESCQIFF
110
GITACLSQVS
120
SEDRSALWAL
130
VTFYGGDCQL
140
TLNKKCTHLI
150
VPEPKGEKYE
160
CALKRASIKI
170
VTPDWVLDCV
180
SEKTKKDEAF
190
YHPRLIIYEE
200
EEEEEEEEEE
210
VENEEQDSQN
220
EGSTDEKSSP
230
ASSQEGSPSG
240
DQQFSPKSNT
250
EKSKGELMFD
260
DSSDSSPEKQ
270
ERNLNWTPAE
280
VPQLAAAKRR
290
LPQGKEPGLI
300
NLCANVPPVP
310
GNILPPEVRG
320
NLMAAGQNLQ
330
SSERSEMIAT
340
WSPAVRTLRN
350
ITNNADIQQM
360
NRPSNVAHIL
370
QTLSAPTKNL
380
EQQVNHSQQG
390
HTNANAVLFS
400
QVKVTPETHM
410
LQQQQQAQQQ
420
QQQHPVLHLQ
430
PQQIMQLQQQ
440
QQQQISQQPY
450
PQQPPHPFSQ
460
QQQQQQQAHP
470
HQFSQQQLQF
480
PQQQLHPPQQ
490
LHRPQQQLQP
500
FQQQHALQQQ
510
FHQLQQHQLQ
520
QQQLAQLQQQ
530
HSLLQQQQQQ
540
QIQQQQLQRM
550
HQQQQQQQMQ
560
SQTAPHLSQT
570
SQALQHQVPP
580
QQPPQQQQQQ
590
QPPPSPQQHQ
600
LFGHDPAVEI
610
PEEGFLLGCV
620
FAIADYPEQM
630
SDKQLLATWK
640
RIIQAHGGTV
650
DPTFTSRCTH
660
LLCESQVSSA
670
YAQAIRERKR
680
CVTAHWLNTV
690
LKKKKMVPPH
700
RALHFPVAFP
710
PGGKPCSQHI
720
ISVTGFVDSD
730
RDDLKLMAYL
740
AGAKYTGYLC
750
RSNTVLICKE
760
PTGLKYEKAK
770
EWRIPCVNAQ
780
WLGDILLGNF
790
EALRQIQYSR
800
YTAFSLQDPF
810
APTQHLVLNL
820
LDAWRVPLKV
830
SAELLMSIRL
840
PPKLKQNEVA
850
NVQPSSKRAR
860
IEDVPPPTKK
870
LTPELTPFVL
880
FTGFEPVQVQ
890
QYIKKLYILG
900
GEVAESAQKC
910
THLIASKVTR
920
TVKFLTAISV
930
VKHIVTPEWL
940
EECFRCQKFI
950
DEQNYILRDA
960
EAEVLFSFSL
970
EESLKRAHVS
980
PLFKAKYFYI
990
TPGICPSLST
1000
MKAIVECAGG
1010
KVLSKQPSFR
1020
KLMEHKQNSS
1030
LSEIILISCE
1040
NDLHLCREYF
1050
ARGIDVHNAE
1060
FVLTGVLTQT
LDYESYKFN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0035097 |
histone methyltransferase complex |
| Cellular Component |
GO:0044666 |
MLL3/4 complex |
| Cellular Component |
GO:0016363 |
nuclear matrix |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Biological Process |
GO:0060612 |
adipose tissue development |
| Biological Process |
GO:0060717 |
chorion development |
| Biological Process |
GO:0006338 |
chromatin remodeling |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0030330 |
DNA damage response, signal transduction by p53 class mediator |
| Biological Process |
GO:0006310 |
DNA recombination |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0043542 |
endothelial cell migration |
| Biological Process |
GO:0045830 |
positive regulation of isotype switching |
| Biological Process |
GO:0048304 |
positive regulation of isotype switching to IgG isotypes |
| Biological Process |
GO:0031398 |
positive regulation of protein ubiquitination |
| Biological Process |
GO:0060261 |
positive regulation of transcription initiation by RNA polymerase II |
| Biological Process |
GO:1902749 |
regulation of cell cycle G2/M phase transition |
| Biological Process |
GO:0010212 |
response to ionizing radiation |
| Biological Process |
GO:0001570 |
vasculogenesis |
Reference
PMID: N/A