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Overview

Uniprot IDQ767K6
Protein NamePre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Gene NameDHX16
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1031 ELEDPHAKKMPKKTG
344 RLGAASLKFGARDAA
702 DPGFCKQKSYNPRTG
93 EARALLEKNRSYRLL

Function

Required for pre-mRNA splicing as a component of the spliceosome. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA. Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses

Protein Sequence

10 MATPAGLERW 20 VQDELHSVLG 30 LSERHVAQFL 40 IGTAQRCASA 50 EEFVQRLRDT 60 DTLDLSGPAR 70 DFALKLWNKV 80 PRKAVAEKPA 90 RAAEREARAL 100 LEKNRSYRLL 110 EDSEESSEEA 120 VGRAGSSLQK 130 KRKKRKHLRK 140 KRQEEEEEEE 150 EEEVPEKGKK 160 TTGGNKPQTE 170 KPESEDEWER 180 TERERLQDLE 190 ERDAFAERVR 200 QRDKDRTRNV 210 LERSDKKAYE 220 EAQKRLKMAE 230 EDRKAMVPEL 240 RKKSRREYLA 250 KREREKLEDL 260 EAELADEEFL 270 FGDVELSRHE 280 RRELKYKRRV 290 RDLAREYRAA 300 GEQEKLEATN 310 RYHMPEETRG 320 QPTRAVDLVE 330 EESGAPGEEQ 340 RRWEEARLGA 350 ASLKFGARDA 360 ASQEPKYQLV 370 LEEEETIEFV 380 RATQLQGDEE 390 PAAPPAPTQA 400 QQKESIQAVR 410 RSLPVFPFRE 420 ELLAAVANHQ 430 ILIIEGETGS 440 GKTTQIPQYL 450 FEEGYTQKGM 460 KIACTQPRRV 470 AAMSVAARVA 480 REMGVKLGNE 490 VGYSIRFEDC 500 TSERTVLRYM 510 TDGMLLREFL 520 SEPDLASYSV 530 VMVDEAHERT 540 LHTDILFGLI 550 KDVARFRPEL 560 KVLVASATLD 570 TARFSTFFDD 580 APVFRIPGRR 590 FPVDIFYTKA 600 PEADYLEACV 610 VSVLQIHVTQ 620 PPGDILVFLT 630 GQEEIEAACE 640 MLQDRCRRLG 650 SKIRELLVLP 660 IYANLPSDMQ 670 ARIFQPTPPG 680 ARKVVVATNI 690 AETSLTIEGI 700 IYVLDPGFCK 710 QKSYNPRTGM 720 ESLTVTPCSK 730 ASANQRAGRA 740 GRVAAGKCFR 750 LYTAWAYQHE 760 LEETTVPEIQ 770 RTSLGNVVLL 780 LKSLGIHDLM 790 HFDFLDPPPY 800 ETLLLALEQL 810 YALGALNHLG 820 ELTTSGRKMA 830 ELPVDPMLSK 840 MILASEKYSC 850 SEEILTVAAM 860 LSVNNSIFYR 870 PKDKVVHADN 880 ARVNFFLPGG 890 DHLVLLNVYT 900 QWAESGYSSQ 910 WCYENFVQFR 920 SMRRARDVRE 930 QLEGLLERVE 940 VGLSSCQGDY 950 IRVRKAITAG 960 YFYHTARLTR 970 SGYRTVKQQQ 980 TVFIHPNSSL 990 FEEQPRWLLY 1000 HELVLTTKEF 1010 MRQVLEIESS 1020 WLLEVAPHYY 1030 KAKELEDPHA 1040 KKMPKKTGKT REELG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0004386 helicase activity
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0038187 pattern recognition receptor activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Molecular Function GO:0043130 ubiquitin binding
Biological Process GO:0140374 antiviral innate immune response
Biological Process GO:0000398 mRNA splicing, via spliceosome

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.