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Overview

Uniprot ID	Q767L8
Protein Name	Mediator of DNA damage checkpoint protein 1
Gene Name	MDC1
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
258	IKDQCPVKEKHKDAR

Function

Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs): TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis. Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs

Protein Sequence

10 MEDTQVINWE 20 AEEEEVEEGP 30 SEPLGCNLQP 40 VGQLRIFSSS 50 YGPEKDFPLY 60 LGKNVVGRMP 70 ECSVALPFSS 80 ISKQHAVIEI 90 LAWNKAPVLR 100 DCGSLNGTQI 110 LRPPKVLGPG 120 VSHRLRNREL 130 ILFADLPCQY 140 HRLDVPPPLV 150 SRGPLTVEET 160 PRGQGGTQPH 170 RLLLAEDSEE 180 EVDSLSERCV 190 VKGPKTSLTT 200 VIPESDEEGP 210 SPAPDGPGPP 220 FAFNLNSDTD 230 EEESQQPGAG 240 EAPSAAETEQ 250 PKPVTTEIQL 260 IKDQCPVKEK 270 HKDARVKRAA 280 SNGVVPVGAI 290 LERSQPAGED 300 SDTDVDEGSG 310 LPRRPAGAHS 320 ERAQPCGFID 330 SDTDAEEEGI 340 PATPAVVPVK 350 KRQIFHEVGT 360 ESPQAPGVAH 370 AQESPAGSDT 380 DIEEGEAPRT 390 VPLDSSRASM 400 MIDSNMDDEE 410 EVSAALTLAR 420 LRESQAVPWN 430 RDAGAGDHRA 440 QPVALLDQSQ 450 ASAGRDSDTD 460 MEEEGLPLEK 470 RGSLPKGPAD 480 KAHPEKSQPP 490 LRGSDVKVEE 500 DERSPGVHPG 510 RSQASATVDA 520 ITQVEEKAPP 530 RPAVTLSEQH 540 QVPVAWTPQT 550 DVEAEGDPAK 560 LPVVHPAEAR 570 PPPAGGHEPD 580 VEKNTSLAAS 590 AGADVRKSQL 600 LAEGDAGTEW 610 AVAILAQDRA 620 LGAGAQSVSA 630 RAQVGQDLPL 640 VSREHLADVA 650 VDTGAPGEAT 660 QTQREGTQAL 670 TERERELNVD 680 RTIDSGDNRD 690 DSEDLDLQAT 700 QCFVEGENQS 710 PEAVPSMEDE 720 PTQAFPSTLP 730 QEPGPSCCSF 740 QASGTLDEPW 750 ELLATQPFCP 760 RESEASEIQP 770 VDTHLEARGL 780 CPSPPRAALP 790 EQHPESPEPL 800 GSQGGGRQTV 810 EKATGTPRET 820 AEGPTPERGP 830 LERATKEPPS 840 EGERGGMGEE 850 GLPRGTQDRE 860 EKQVLAGVTQ 870 RQESDRTVKS 880 TSTDGGLESL 890 QVEIETPKEM 900 QENEIEKQTL 910 ARDMLEREAE 920 KPVAERESEA 930 GGPEVKVPEA 940 VQDRGPLRAE 950 AEGTSQDQKG 960 QASNLTPEPG 970 AGVGYLQGLA 980 SAPAAPRSQA 990 GGGGEAPVSP 1000 RRQQRGDLNC 1010 KMPPAEKSGV 1020 RAAFSPSLPS 1030 PHGDQESPEA 1040 CLPPEPPEAS 1050 APLQNPLPSQ 1060 SPKHPAPQSR 1070 LSPPPPPLEQ 1080 STPRTRPPQS 1090 QESPEPPFSS 1100 ELDPPNPEPK 1110 VRPQGSPPLS 1120 PIPLEAHPTS 1130 PTDQAGSPEP 1140 TSRAARGGTH 1150 RSFEVTPMSV 1160 VPTALELQSS 1170 TSADQPVVPK 1180 PTLRAPWGRT 1190 HRSSVKTPEP 1200 NIPTAPELQP 1210 STPTDQPVAP 1220 EPLSRATRGR 1230 TPRASVKTSE 1240 PVVPAAPEPQ 1250 PSTPTDQPVV 1260 PKPTLRAPRG 1270 RTHRSSVKTP 1280 EPNIPTAPEL 1290 RPSTPTDQPV 1300 APEPLSRATR 1310 GRTPRASVKS 1320 PEQNVPTAPE 1330 HPQPSTPTDQ 1340 PVTPKPTSRA 1350 TRGRAHRSSV 1360 KTPAASEPLP 1370 SASTDQPITP 1380 KPTSRGRAHR 1390 SSAKTPELQP 1400 PTSTGQPVTP 1410 RPTSQATRGR 1420 THRSSIKTPE 1430 PVVPTDPEPQ 1440 PSTPTDQPIT 1450 PELTSAATRG 1460 RTRRSSVKTP 1470 EPVVPTDPEP 1480 QPSTPTDQPI 1490 TPKPTSWATR 1500 GQAHRSSVKT 1510 PEPHVPTDPE 1520 PQPSTPTDQP 1530 VTPKPTSRAT 1540 RGRARKSSVK 1550 TPEPAVPTAA 1560 EPQPSASTDQ 1570 PITPKPTSRG 1580 RAHRSSAKTP 1590 ELQPPTSTGQ 1600 PFTPRPTSGA 1610 TQGRTHRSSA 1620 KTSQAVEPTA 1630 PDLEPPSPIG 1640 QPVTPKVIAE 1650 GGQNRILRSS 1660 RVGAVPGPTP 1670 PELQCPVPTE 1680 QPVPPEPIPR 1690 ASCSRRPRAT 1700 RKRESLTAHV 1710 GPDPCSAPPE 1720 PNSRSSRTQS 1730 LSTTPEPTLP 1740 QLPEAPAHAP 1750 QIPKVEAAGR 1760 PGFTLEPQPK 1770 ATQKRKRPLA 1780 PADSPPLPKR 1790 LQRGEVPPKT 1800 VILEEEENPT 1810 ARPGREEDAV 1820 IPEPGKRKRD 1830 QTEEEPRGVP 1840 SRSLRRTKPA 1850 QESTAPRVLF 1860 TGVVDARGER 1870 AVLALGGSLA 1880 SSVAEASHLV 1890 TDRIRRTVKF 1900 LCALGRGIPI 1910 LSLDWLHQSR 1920 KAGCFLPPDE 1930 YVVTDPEQEK 1940 NFGFSLREAL 1950 SRARERKLLE 1960 GYEIHVTPGV 1970 QPPPPQMGEI 1980 ISCCGGTVLP 1990 SMPRSYKPQR 2000 VVITCSQDFP 2010 RCSVPFRLGL 2020 PVLSPEFLLT 2030 GVLKQEAKPE 2040 AFVLSTLEMA SA

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005694	chromosome
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0035861	site of double-strand break
Molecular Function	GO:0140463	chromatin-protein adaptor activity
Molecular Function	GO:0140566	histone reader activity
Biological Process	GO:0006974	DNA damage response
Biological Process	GO:0006281	DNA repair
Biological Process	GO:0000076	DNA replication checkpoint signaling
Biological Process	GO:1990166	protein localization to site of double-strand break

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.