Overview
| Uniprot ID | Q7L0Y3 |
|---|
| Protein Name | tRNA methyltransferase 10 homolog C |
|---|
| Gene Name | TRMT10C |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 305 |
MTTFRHDKVYVIGSF |
| 325 |
QPGTSLAKAKRLNLA |
Function
Mitochondrial tRNA N(1)-methyltransferase involved in mitochondrial tRNA maturation (PubMed:18984158, PubMed:21593607, PubMed:23042678, PubMed:27132592). Component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends (PubMed:18984158). Together with HSD17B10/MRPP2, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity (PubMed:23042678, PubMed:29040705). The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)-methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs; TRMT10C/MRPP1 acting as the catalytic N(1)-methyltransferase subunit (PubMed:23042678). The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme (PubMed:29040705). In addition to tRNA N(1)-methyltransferase activity, TRMT10C/MRPP1 also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA (PubMed:29072297). Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly
Protein Sequence
10
MAAFLKMSVS
20
VNFFRPFTRF
30
LVPFTLHRKR
40
NNLTILQRYM
50
SSKIPAVTYP
60
KNESTPPSEE
70
LELDKWKTTM
80
KSSVQEECVS
90
TISSSKDEDP
100
LAATREFIEM
110
WRLLGREVPE
120
HITEEELKTL
130
MECVSNTAKK
140
KYLKYLYTKE
150
KVKKARQIKK
160
EMKAAAREEA
170
KNIKLLETTE
180
EDKQKNFLFL
190
RLWDRNMDIA
200
MGWKGAQAMQ
210
FGQPLVFDMA
220
YENYMKRKEL
230
QNTVSQLLES
240
EGWNRRNVDP
250
FHIYFCNLKI
260
DGALHRELVK
270
RYQEKWDKLL
280
LTSTEKSHVD
290
LFPKDSIIYL
300
TADSPNVMTT
310
FRHDKVYVIG
320
SFVDKSMQPG
330
TSLAKAKRLN
340
LATECLPLDK
350
YLQWEIGNKN
360
LTLDQMIRIL
370
LCLKNNGNWQ
380
EALQFVPKRK
390
HTGFLEISQH
400
SQEFINRLKK
AKT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0042645 |
mitochondrial nucleoid |
| Cellular Component |
GO:0030678 |
mitochondrial ribonuclease P complex |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0160106 |
tRNA (adenine(9)-N1)-methyltransferase activity |
| Molecular Function |
GO:0052905 |
tRNA (guanosine(9)-N1)-methyltransferase activity |
| Molecular Function |
GO:0000049 |
tRNA binding |
| Biological Process |
GO:0000964 |
mitochondrial RNA 5'-end processing |
| Biological Process |
GO:1990180 |
mitochondrial tRNA 3'-end processing |
| Biological Process |
GO:0097745 |
mitochondrial tRNA 5'-end processing |
| Biological Process |
GO:0070901 |
mitochondrial tRNA methylation |
| Biological Process |
GO:0090646 |
mitochondrial tRNA processing |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0070131 |
positive regulation of mitochondrial translation |
| Biological Process |
GO:0042780 |
tRNA 3'-end processing |
| Biological Process |
GO:0001680 |
tRNA 3'-terminal CCA addition |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.