Search Results

Overview

Uniprot IDQ7Z2T5
Protein NametRNA (guanine(27)-N(2))-dimethyltransferase
Gene NameTRMT1L
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
324 QENCHLNKLKVVVDS
326 NCHLNKLKVVVDSKE
609 DNYIAQGKRKSNEMI
611 YIAQGKRKSNEMITN

Function

Specifically dimethylates a single guanine residue at position 27 of tRNA(Tyr) using S-adenosyl-L-methionine as donor of the methyl groups (PubMed:39786990, PubMed:39786998). Dimethylation at position 27 of tRNA(Tyr) is required for efficient translation of tyrosine codons (PubMed:39786990, PubMed:39786998). Also required to maintain 3-(3-amino-3-carboxypropyl)uridine (acp3U) in the D-loop of several cytoplasmic tRNAs (PubMed:39786990, PubMed:39786998)

Protein Sequence

10 MENMAEEELL 20 PLEKEEVEVA 30 QVQVPTPARD 40 SAGVPAPAPD 50 SALDSAPTPA 60 SAPAPAPALA 70 QAPALSPSLA 80 SAPEEAKSKR 90 HISIQRQLAD 100 LENLAFVTDG 110 NFDSASSLNS 120 DNLDAGNRQA 130 CPLCPKEKFR 140 ACNSHKLRRH 150 LQNLHWKVSV 160 EFEGYRMCIC 170 HLPCRPVKPN 180 IIGEQITSKM 190 GAHYHCIICS 200 ATITRRTDML 210 GHVRRHMNKG 220 ETKSSYIAAS 230 TAKPPKEILK 240 EADTDVQVCP 250 NYSIPQKTDS 260 YFNPKMKLNR 270 QLIFCTLAAL 280 AEERKPLECL 290 DAFGATGIMG 300 LQWAKHLGNA 310 VKVTINDLNE 320 NSVTLIQENC 330 HLNKLKVVVD 340 SKEKEKSDDI 350 LEEGEKNLGN 360 IKVTKMDANV 370 LMHLRSFDFI 380 HLDPFGTSVN 390 YLDSAFRNIR 400 NLGIVSVTST 410 DISSLYAKAQ 420 HVARRHYGCN 430 IVRTEYYKEL 440 AARIVVAAVA 450 RAAARCNKGI 460 EVLFAVALEH 470 FVLVVVRVLR 480 GPTSADETAK 490 KIQYLIHCQW 500 CEERIFQKDG 510 NMVEENPYRQ 520 LPCNCHGSMP 530 GKTAIELGPL 540 WSSSLFNTGF 550 LKRMLFESLH 560 HGLDDIQTLI 570 KTLIFESECT 580 PQSQFSIHAS 590 SNVNKQEENG 600 VFIKTTDDTT 610 TDNYIAQGKR 620 KSNEMITNLG 630 KKQKTDVSTE 640 HPPFYYNIHR 650 HSIKGMNMPK 660 LKKFLCYLSQ 670 AGFRVSRTHF 680 DPMGVRTDAP 690 LMQFKSILLK 700 YSTPTYTGGQ 710 SESHVQSASE 720 DTVTERVEMS 730 VNDKAEASGC RRW

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0160248 tRNA (guanine(27)-N2)-dimethyltransferase activity
Molecular Function GO:0016423 tRNA (guanine) methyltransferase activity
Molecular Function GO:0000049 tRNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0002940 tRNA N2-guanine methylation
Biological Process GO:0008033 tRNA processing

Reference

[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.