Search Results

Overview

Uniprot IDQ7Z333
Protein NameHelicase senataxin
Gene NameSETX
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1225 VSQKKSSKLCTCTEP
1240 IRKVPVSKTPKKTHS
1273 PAIVPPKKFRQCPEP
2352 ITHYKAQKTMIQKDL
2672 EQEDSSSKKRKLL**
2673 QEDSSSKKRKLL***
774 LKDDALAKTSKRKTK
784 KRKTKVQKDEICAKL
790 QKDEICAKLSHVIKK
827 IESFYSRKDTGVQKG

Function

ATP-dependent 5'-3' helicase that preferentially unwinds RNA:DNA substrates over DNA:DNA substrates, playing a crucial role in resolving 5'-overhang RNA:DNA hybrids (R-loops) and promoting transcription termination (PubMed:36864660). Plays a role in transcription regulation by its ability to modulate RNA Polymerase II (Pol II) binding to chromatin and through its interaction with proteins involved in transcription (PubMed:19515850, PubMed:21700224). Contributes to the mRNA splicing efficiency and splice site selection (PubMed:19515850). Required for the resolution of R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site, allowing XRN2 recruitment and XRN2-mediated degradation of the downstream cleaved RNA and hence efficient RNA polymerase II (RNAp II) transcription termination (PubMed:19515850, PubMed:21700224, PubMed:26700805). Required for the 3' transcriptional termination of PER1 and CRY2, thus playing an important role in the circadian rhythm regulation (By similarity). Involved in DNA double-strand breaks damage response generated by oxidative stress (PubMed:17562789). In association with RRP45, targets the RNA exosome complex to sites of transcription-induced DNA damage (PubMed:24105744). Plays a role in the development and maturation of germ cells: essential for male meiosis, acting at the interface of transcription and meiotic recombination, and in the process of gene silencing during meiotic sex chromosome inactivation (MSCI) (By similarity). May be involved in telomeric stability through the regulation of telomere repeat-containing RNA (TERRA) transcription (PubMed:21112256). Plays a role in neurite outgrowth in hippocampal cells through FGF8-activated signaling pathways. Inhibits retinoic acid-induced apoptosis (PubMed:21576111)

Protein Sequence

10 MSTCCWCTPG 20 GASTIDFLKR 30 YASNTPSGEF 40 QTADEDLCYC 50 LECVAEYHKA 60 RDELPFLHEV 70 LWELETLRLI 80 NHFEKSMKAE 90 IGDDDELYIV 100 DNNGEMPLFD 110 ITGQDFENKL 120 RVPLLEILKY 130 PYLLLHERVN 140 ELCVEALCRM 150 EQANCSFQVF 160 DKHPGIYLFL 170 VHPNEMVRRW 180 AILTARNLGK 190 VDRDDYYDLQ 200 EVLLCLFKVI 210 ELGLLESPDI 220 YTSSVLEKGK 230 LILLPSHMYD 240 TTNYKSYWLG 250 ICMLLTILEE 260 QAMDSLLLGS 270 DKQNDFMQSI 280 LHTMEREADD 290 DSVDPFWPAL 300 HCFMVILDRL 310 GSKVWGQLMD 320 PIVAFQTIIN 330 NASYNREIRH 340 IRNSSVRTKL 350 EPESYLDDMV 360 TCSQIVYNYN 370 PEKTKKDSGW 380 RTAICPDYCP 390 NMYEEMETLA 400 SVLQSDIGQD 410 MRVHNSTFLW 420 FIPFVQSLMD 430 LKDLGVAYIA 440 QVVNHLYSEV 450 KEVLNQTDAV 460 CDKVTEFFLL 470 ILVSVIELHR 480 NKKCLHLLWV 490 SSQQWVEAVV 500 KCAKLPTTAF 510 TRSSEKSSGN 520 CSKGTAMISS 530 LSLHSMPSNS 540 VQLAYVQLIR 550 SLLKEGYQLG 560 QQSLCKRFWD 570 KLNLFLRGNL 580 SLGWQLTSQE 590 THELQSCLKQ 600 IIRNIKFKAP 610 PCNTFVDLTS 620 ACKISPASYN 630 KEESEQMGKT 640 SRKDMHCLEA 650 SSPTFSKEPM 660 KVQDSVLIKA 670 DNTIEGDNNE 680 QNYIKDVKLE 690 DHLLAGSCLK 700 QSSKNIFTER 710 AEDQIKISTR 720 KQKSVKEISS 730 YTPKDCTSRN 740 GPERGCDRGI 750 IVSTRLLTDS 760 STDALEKVST 770 SNEDFSLKDD 780 ALAKTSKRKT 790 KVQKDEICAK 800 LSHVIKKQHR 810 KSTLVDNTIN 820 LDENLTVSNI 830 ESFYSRKDTG 840 VQKGDGFIHN 850 LSLDPSGVLD 860 DKNGEQKSQN 870 NVLPKEKQLK 880 NEELVIFSFH 890 ENNCKIQEFH 900 VDGKELIPFT 910 EMTNASEKKS 920 SPFKDLMTVP 930 ESRDEEMSNS 940 TSVIYSNLTR 950 EQAPDISPKS 960 DTLTDSQIDR 970 DLHKLSLLAQ 980 ASVITFPSDS 990 PQNSSQLQRK 1000 VKEDKRCFTA 1010 NQNNVGDTSR 1020 GQVIIISDSD 1030 DDDDERILSL 1040 EKLTKQDKIC 1050 LEREHPEQHV 1060 STVNSKEEKN 1070 PVKEEKTETL 1080 FQFEESDSQC 1090 FEFESSSEVF 1100 SVWQDHPDDN 1110 NSVQDGEKKC 1120 LAPIANTTNG 1130 QGCTDYVSEV 1140 VKKGAEGIEE 1150 HTRPRSISVE 1160 EFCEIEVKKP 1170 KRKRSEKPMA 1180 EDPVRPSSSV 1190 RNEGQSDTNK 1200 RDLVGNDFKS 1210 IDRRTSTPNS 1220 RIQRATTVSQ 1230 KKSSKLCTCT 1240 EPIRKVPVSK 1250 TPKKTHSDAK 1260 KGQNRSSNYL 1270 SCRTTPAIVP 1280 PKKFRQCPEP 1290 TSTAEKLGLK 1300 KGPRKAYELS 1310 QRSLDYVAQL 1320 RDHGKTVGVV 1330 DTRKKTKLIS 1340 PQNLSVRNNK 1350 KLLTSQELQM 1360 QRQIRPKSQK 1370 NRRRLSDCES 1380 TDVKRAGSHT 1390 AQNSDIFVPE 1400 SDRSDYNCTG 1410 GTEVLANSNR 1420 KQLIKCMPSE 1430 PETIKAKHGS 1440 PATDDACPLN 1450 QCDSVVLNGT 1460 VPTNEVIVST 1470 SEDPLGGGDP 1480 TARHIEMAAL 1490 KEGEPDSSSD 1500 AEEDNLFLTQ 1510 NDPEDMDLCS 1520 QMENDNYKLI 1530 ELIHGKDTVE 1540 VEEDSVSRPQ 1550 LESLSGTKCK 1560 YKDCLETTKN 1570 QGEYCPKHSE 1580 VKAADEDVFR 1590 KPGLPPPASK 1600 PLRPTTKIFS 1610 SKSTSRIAGL 1620 SKSLETSSAL 1630 SPSLKNKSKG 1640 IQSILKVPQP 1650 VPLIAQKPVG 1660 EMKNSCNVLH 1670 PQSPNNSNRQ 1680 GCKVPFGESK 1690 YFPSSSPVNI 1700 LLSSQSVSDT 1710 FVKEVLKWKY 1720 EMFLNFGQCG 1730 PPASLCQSIS 1740 RPVPVRFHNY 1750 GDYFNVFFPL 1760 MVLNTFETVA 1770 QEWLNSPNRE 1780 NFYQLQVRKF 1790 PADYIKYWEF 1800 AVYLEECELA 1810 KQLYPKENDL 1820 VFLAPERINE 1830 EKKDTERNDI 1840 QDLHEYHSGY 1850 VHKFRRTSVM 1860 RNGKTECYLS 1870 IQTQENFPAN 1880 LNELVNCIVI 1890 SSLVTTQRKL 1900 KAMSLLGSRN 1910 QLARAVLNPN 1920 PMDFCTKDLL 1930 TTTSERIIAY 1940 LRDFNEDQKK 1950 AIETAYAMVK 1960 HSPSVAKICL 1970 IHGPPGTGKS 1980 KTIVGLLYRL 1990 LTENQRKGHS 2000 DENSNAKIKQ 2010 NRVLVCAPSN 2020 AAVDELMKKI 2030 ILEFKEKCKD 2040 KKNPLGNCGD 2050 INLVRLGPEK 2060 SINSEVLKFS 2070 LDSQVNHRMK 2080 KELPSHVQAM 2090 HKRKEFLDYQ 2100 LDELSRQRAL 2110 CRGGREIQRQ 2120 ELDENISKVS 2130 KERQELASKI 2140 KEVQGRPQKT 2150 QSIIILESHI 2160 ICCTLSTSGG 2170 LLLESAFRGQ 2180 GGVPFSCVIV 2190 DEAGQSCEIE 2200 TLTPLIHRCN 2210 KLILVGDPKQ 2220 LPPTVISMKA 2230 QEYGYDQSMM 2240 ARFCRLLEEN 2250 VEHNMISRLP 2260 ILQLTVQYRM 2270 HPDICLFPSN 2280 YVYNRNLKTN 2290 RQTEAIRCSS 2300 DWPFQPYLVF 2310 DVGDGSERRD 2320 NDSYINVQEI 2330 KLVMEIIKLI 2340 KDKRKDVSFR 2350 NIGIITHYKA 2360 QKTMIQKDLD 2370 KEFDRKGPAE 2380 VDTVDAFQGR 2390 QKDCVIVTCV 2400 RANSIQGSIG 2410 FLASLQRLNV 2420 TITRAKYSLF 2430 ILGHLRTLME 2440 NQHWNQLIQD 2450 AQKRGAIIKT 2460 CDKNYRHDAV 2470 KILKLKPVLQ 2480 RSLTHPPTIA 2490 PEGSRPQGGL 2500 PSSKLDSGFA 2510 KTSVAASLYH 2520 TPSDSKEITL 2530 TVTSKDPERP 2540 PVHDQLQDPR 2550 LLKRMGIEVK 2560 GGIFLWDPQP 2570 SSPQHPGATP 2580 PTGEPGFPVV 2590 HQDLSHIQQP 2600 AAVVAALSSH 2610 KPPVRGEPPA 2620 ASPEASTCQS 2630 KCDDPEEELC 2640 HRREARAFSE 2650 GEQEKCGSET 2660 HHTRRNSRWD 2670 KRTLEQEDSS SKKRKLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030424 axon
Cellular Component GO:0000781 chromosome, telomeric region
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0030426 growth cone
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0000228 nuclear chromosome
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0033677 DNA/RNA helicase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Molecular Function GO:0001147 transcription termination site sequence-specific DNA binding
Biological Process GO:0030154 cell differentiation
Biological Process GO:0070301 cellular response to hydrogen peroxide
Biological Process GO:0034599 cellular response to oxidative stress
Biological Process GO:0006974 DNA damage response
Biological Process GO:0006310 DNA recombination
Biological Process GO:0006353 DNA-templated transcription termination
Biological Process GO:0006302 double-strand break repair
Biological Process GO:0006376 mRNA splice site recognition
Biological Process GO:0007399 nervous system development
Biological Process GO:2000144 positive regulation of DNA-templated transcription initiation
Biological Process GO:0010976 positive regulation of neuron projection development
Biological Process GO:0033120 positive regulation of RNA splicing
Biological Process GO:0060566 positive regulation of termination of DNA-templated transcription
Biological Process GO:2000806 positive regulation of termination of RNA polymerase II transcription, poly(A)-coupled
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0048511 rhythmic process
Biological Process GO:0006396 RNA processing
Biological Process GO:0007283 spermatogenesis
Biological Process GO:0006369 termination of RNA polymerase II transcription

Reference

[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.