Search Results

Overview

Uniprot IDQ7Z5K2
Protein NameWings apart-like protein homolog
Gene NameWAPL
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
27 FDEVFSNKRTTLSTK
313 QGASNFDKLMDGTSQ
331 KANSESSKDGLNQAK
339 DGLNQAKKGGVSCGT
401 EAGRLRKKADIATSK
408 KADIATSKTTTRFRP
43 GETTFMAKLGQKRPN
541 KSKENTRKIFSGPKR
547 RKIFSGPKRSPTKAV
552 GPKRSPTKAVYNARH
586 VTVRLSSKEPNQKDD
591 SSKEPNQKDDGVFKA
597 QKDDGVFKAPAPPSK
604 KAPAPPSKVIKTVTI
624 QDIVTALKCRREDKE
758 EQDASSAKLLNEKDM

Function

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin (PubMed:26299517). Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair

Protein Sequence

10 MTSRFGKTYS 20 RKGGNGSSKF 30 DEVFSNKRTT 40 LSTKWGETTF 50 MAKLGQKRPN 60 FKPDIQEIPK 70 KPKVEEESTG 80 DPFGFDSDDE 90 SLPVSSKNLA 100 QVKCSSYSES 110 SEAAQLEEVT 120 SVLEANSKIS 130 HVVVEDTVVS 140 DKCFPLEDTL 150 LGKEKSTNRI 160 VEDDASISSC 170 NKLITSDKVE 180 NFHEEHEKNS 190 HHIHKNADDS 200 TKKPNAETTV 210 ASEIKETNDT 220 WNSQFGKRPE 230 SPSEISPIKG 240 SVRTGLFEWD 250 NDFEDIRSED 260 CILSLDSDPL 270 LEMKDDDFKN 280 RLENLNEAIE 290 EDIVQSVLRP 300 TNCRTYCRAN 310 KTKSSQGASN 320 FDKLMDGTSQ 330 ALAKANSESS 340 KDGLNQAKKG 350 GVSCGTSFRG 360 TVGRTRDYTV 370 LHPSCLSVCN 380 VTIQDTMERS 390 MDEFTASTPA 400 DLGEAGRLRK 410 KADIATSKTT 420 TRFRPSNTKS 430 KKDVKLEFFG 440 FEDHETGGDE 450 GGSGSSNYKI 460 KYFGFDDLSE 470 SEDDEDDDCQ 480 VERKTSKKRT 490 KTAPSPSLQP 500 PPESNDNSQD 510 SQSGTNNAEN 520 LDFTEDLPGV 530 PESVKKPINK 540 QGDKSKENTR 550 KIFSGPKRSP 560 TKAVYNARHW 570 NHPDSEELPG 580 PPVVKPQSVT 590 VRLSSKEPNQ 600 KDDGVFKAPA 610 PPSKVIKTVT 620 IPTQPYQDIV 630 TALKCRREDK 640 ELYTVVQHVK 650 HFNDVVEFGE 660 NQEFTDDIEY 670 LLSGLKSTQP 680 LNTRCLSVIS 690 LATKCAMPSF 700 RMHLRAHGMV 710 AMVFKTLDDS 720 QHHQNLSLCT 730 AALMYILSRD 740 RLNMDLDRAS 750 LDLMIRLLEL 760 EQDASSAKLL 770 NEKDMNKIKE 780 KIRRLCETVH 790 NKHLDLENIT 800 TGHLAMETLL 810 SLTSKRAGDW 820 FKEELRLLGG 830 LDHIVDKVKE 840 CVDHLSRDED 850 EEKLVASLWG 860 AERCLRVLES 870 VTVHNPENQS 880 YLIAYKDSQL 890 IVSSAKALQH 900 CEELIQQYNR 910 AEDSICLADS 920 KPLPHQNVTN 930 HVGKAVEDCM 940 RAIIGVLLNL 950 TNDNEWGSTK 960 TGEQDGLIGT 970 ALNCVLQVPK 980 YLPQEQRFDI 990 RVLGLGLLIN 1000 LVEYSARNRH 1010 CLVNMETSCS 1020 FDSSICSGEG 1030 DDSLRIGGQV 1040 HAVQALVQLF 1050 LERERAAQLA 1060 ESKTDELIKD 1070 APTTQHDKSG 1080 EWQETSGEIQ 1090 WVSTEKTDGT 1100 EEKHKKEEED 1110 EELDLNKALQ 1120 HAGKHMEDCI 1130 VASYTALLLG 1140 CLCQESPINV 1150 TTVREYLPEG 1160 DFSIMTEMLK 1170 KFLSFMNLTC 1180 AVGTTGQKSI 1190 SRVIEYLEHC

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005694 chromosome
Cellular Component GO:0000775 chromosome, centromeric region
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0042585 germinal vesicle
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000795 synaptonemal complex
Molecular Function GO:0140083 ATP-dependent protein-DNA unloader activity
Biological Process GO:0051301 cell division
Biological Process GO:0000070 mitotic sister chromatid segregation
Biological Process GO:0035562 negative regulation of chromatin binding
Biological Process GO:0008156 negative regulation of DNA replication
Biological Process GO:0045875 negative regulation of sister chromatid cohesion
Biological Process GO:0071168 protein localization to chromatin

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[8] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.