Overview
| Uniprot ID | Q7Z6J9 |
|---|
| Protein Name | tRNA-splicing endonuclease subunit Sen54 |
|---|
| Gene Name | TSEN54 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 215 |
RSINKKAKALDNSLQ |
Function
Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events
Protein Sequence
10
MEPEPEPAAV
20
EVPAGRVLSA
30
RELFAARSRS
40
QKLPQRSHGP
50
KDFLPDGSAA
60
QAERLRRCRE
70
ELWQLLAEQR
80
VERLGSLVAA
90
EWRPEEGFVE
100
LKSPAGKFWQ
110
TMGFSEQGRQ
120
RLHPEEALYL
130
LECGSIHLFH
140
QDLPLSIQEA
150
YQLLLTDHTV
160
TFLQYQVFSH
170
LKRLGYVVRR
180
FQPSSVLSPY
190
ERQLNLDASV
200
QHLEDGDGKR
210
KRSSSSPRSI
220
NKKAKALDNS
230
LQPKSLAASS
240
PPPCSQPSQC
250
PEEKPQESSP
260
MKGPGGPFQL
270
LGSLGPSPGP
280
AREGVGCSWE
290
SGRAENGVTG
300
AGKRRWNFEQ
310
ISFPNMASDS
320
RHTLLRAPAP
330
ELLPANVAGR
340
ETDAESWCQK
350
LNQRKEKLSR
360
REREHHAEAA
370
QFQEDVNADP
380
EVQRCSSWRE
390
YKELLQRRQV
400
QRSQRRAPHL
410
WGQPVTPLLS
420
PGQASSPAVV
430
LQHISVLQTT
440
HLPDGGARLL
450
EKSGGLEIIF
460
DVYQADAVAT
470
FRKNNPGKPY
480
ARMCISGFDE
490
PVPDLCSLKR
500
LSYQSGDVPL
510
IFALVDHGDI
520
SFYSFRDFTL
PQDVGH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005730 |
nucleolus |
| Biological Process |
GO:0006388 |
tRNA splicing, via endonucleolytic cleavage and ligation |
| Biological Process |
GO:0000379 |
tRNA-type intron splice site recognition and cleavage |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0000214 |
tRNA-intron endonuclease complex |
| Biological Process |
GO:0006397 |
mRNA processing |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.